Proteomics

Dataset Information

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A SWATH LABEL-FREE PROTEOMICS INSIGHT INTO THE FAAH-/- MOUSE LIVER

ABSTRACT: Fatty acid amide hydrolase (FAAH) is an important enzyme for lipid metabolism and an interesting pharmacological target, given its role in anandamide breakdown. The FAAH-/- genotype is the most widely used mouse model to achieve complete abolition of this enzyme. In this paper we explore, by means of label-free SWATH proteomics, the changes in protein expression occurring in the liver of FAAH-/- mice. We identified several altered biological processes and pathways, like fatty acid synthesis and glycolysis, which explain the observed phenotype of this mouse. We also observed the alteration of other proteins, like carboxylesterases and S-methyltransferases, apparently not immediately related to FAAH, but known to have important biological roles.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Liver

SUBMITTER: Andrea Armirotti  

LAB HEAD: Andrea Armirotti

PROVIDER: PXD010087 | Pride | 2018-08-20

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
MouseLiverBrainIonLibrary_NoMods_1FDR.txt Txt
PM.xml Xml
SWATHResults.xlsx Xlsx
ZH_12Mar2018_LiverFAAH_SWATH_KO1.wiff Wiff
ZH_12Mar2018_LiverFAAH_SWATH_KO1.wiff.1.idx2 Wiff
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Publications

A Swath Label-Free Proteomics insight into the Faah<sup>-/-</sup> Mouse Liver.

Hamid Zeeshan Z   Summa Maria M   Armirotti Andrea A  

Scientific reports 20180814 1

Fatty acid amide hydrolase (FAAH) is an important enzyme for lipid metabolism and an interesting pharmacological target, given its role in anandamide breakdown. The FAAH<sup>-/-</sup> genotype is the most widely used mouse model to investigate the effects of a complete pharmacological inhibition of this enzyme. In this paper, we explore, by means of label-free SWATH proteomics, the changes in protein expression occurring in the liver of FAAH<sup>-/-</sup> knockout (KO) mice. We identified severa  ...[more]

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