Dataset Information

Sucrose metabolism in haloarchaea: reassessment using genomics, proteomics and metagenomics

ABSTRACT: We used quantitative proteomics (8-plex iTRAQ labeling) to investigate pathways and enzymes potentially important for sucrose metabolism in the halophilic archaeon Halohasta litchfieldiae. The data provided insights into the dynamics of the Hht. litchfieldiae proteome in response to sucrose, advancing the understanding of sucrose and fructose metabolism in haloarchaea.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Halohasta Litchfieldiae

SUBMITTER: Timothy Williams

LAB HEAD: Ricardo Cavicchioli

PROVIDER: PXD010137 | Pride | 2019-01-31

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Combine_tADL_CP_Exp1TR1TR2_Exp2TR1TR2.xml	Xml
	Combine_tADL_SUP_Exp1TR1TR2_Exp2TR1TR2.xml	Xml
	tADL_CP_Exp1TR1TR2.xml	Xml
	tADL_CP_Exp1_TR1.wiff	Wiff
	tADL_CP_Exp1_TR1.wiff.scan	Wiff

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Sucrose Metabolism in Haloarchaea: Reassessment Using Genomics, Proteomics, and Metagenomics.

Williams Timothy J TJ Allen Michelle A MA Liao Yan Y Raftery Mark J MJ Cavicchioli Ricardo R

Applied and environmental microbiology 20190306 6

The canonical pathway for sucrose metabolism in haloarchaea utilizes a modified Embden-Meyerhof-Parnas pathway (EMP), in which ketohexokinase and 1-phosphofructokinase phosphorylate fructose released from sucrose hydrolysis. However, our survey of haloarchaeal genomes determined that ketohexokinase and 1-phosphofructokinase genes were not present in all species known to utilize fructose and sucrose, thereby indicating that alternative mechanisms exist for fructose metabolism. A fructokinase gene ...[more]

PMID: 30658981

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