Proteomics

Dataset Information

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Sperm proteome of apyrene and eupyrene sperm in Carolina Sphinx moth Manduca sexta, and monarch butterfly Danaus plexippus


ABSTRACT: All but the most basal Lepidopteran species produce two sperm morphs. Only one of these morphs is capable of completing karyogamy and producing offspring, this morph contains the correct genetic complement and is termed eupyrene sperm. Apyrene sperm, on the other hand, is completely devoid of nuclear DNA and fertilisation incompetent. Despite the fact apyrene sperm is believed to be functional, the function of this sperm type is largely unknown. Here we apply tandem mass spectrometry based proteomics to the two sperm types independently in the monarch butterfly (Danaus plexippus) and the carolina sphinx moth (Manduca sexta). Comparative analysis between sperm morphs and species shows a reduced complexity and greater divergence in apyrene sperm relative to eupyrene consistent across the two species.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Manduca Sexta (tobacco Hawkmoth) (tobacco Hornworm) Danaus Plexippus

TISSUE(S): Sperm

SUBMITTER: Emma Whittington  

LAB HEAD: Dr Steve Dorus

PROVIDER: PXD010168 | Pride | 2019-05-06

REPOSITORIES: pride

Dataset's files

Source:
Action DRS
Manduca_Apyrene_1_1.raw Raw
Manduca_Apyrene_1_2.raw Raw
Manduca_Apyrene_1_3.raw Raw
Manduca_Apyrene_1_4.raw Raw
Manduca_Apyrene_2_1.raw Raw
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Publications

Evolutionary Proteomics Reveals Distinct Patterns of Complexity and Divergence between Lepidopteran Sperm Morphs.

Whittington Emma E   Karr Timothy L TL   Mongue Andrew J AJ   Dorus Steve S   Walters James R JR  

Genome biology and evolution 20190701 7


Spermatozoa are one of the most strikingly diverse animal cell types. One poorly understood example of this diversity is sperm heteromorphism, where males produce multiple distinct morphs of sperm in a single ejaculate. Typically, only one morph is capable of fertilization and the function of the nonfertilizing morph, called parasperm, remains to be elucidated. Sperm heteromorphism has multiple independent origins, including Lepidoptera (moths and butterflies), where males produce a fertilizing  ...[more]

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