Proteomics

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Combined sperm proteome of the monarch butterfly Danaus plexippus

ABSTRACT: We characterized sperm from the seminal vesicles of male monarch butterflies (Danaus plexippus), in triplicate, identifying 548 high confidence proteins. As with all but the most basal lepidopteran species male monarch butterflies are sperm heteromorphic, producing fertilization competent and anucleate fertilization incompetent sperm morphs. Comparing this data to the sperm proteomes of the Carolina sphinx moth (Manduca sexta) and the fruit fly (Drosophila melanogaster) demonstrated high levels of functional coherence across proteomes, and conservation at the level of protein abundance and post-translational modification within Lepidoptera. Comparative genomic analyses revealed a significant reduction in orthology among Monarch sperm genes relative to the remainder of the genome in non-Lepidopteran insects. A substantial number of sperm proteins were found to be specific to Lepidoptera, lacking detectable homology outside this taxa. These findings are consistent with a burst of genetic novelty in the sperm proteome concurrent with the origin of heteromorphic spermatogenesis early in Lepidoptera evolution.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Danaus Plexippus

TISSUE(S): Sperm

SUBMITTER: Emma Whittington  

LAB HEAD: Dr Steve Dorus

PROVIDER: PXD006454 | Pride | 2017-10-26

REPOSITORIES: Pride

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Sperm_AE_1_1.raw Raw
Sperm_AE_1_2.raw Raw
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Publications

Contrasting patterns of evolutionary constraint and novelty revealed by comparative sperm proteomic analysis in Lepidoptera.

Whittington Emma E   Forsythe Desiree D   Borziak Kirill K   Karr Timothy L TL   Walters James R JR   Dorus Steve S  

BMC genomics 20171202 1

<h4>Background</h4>Rapid evolution is a hallmark of reproductive genetic systems and arises through the combined processes of sequence divergence, gene gain and loss, and changes in gene and protein expression. While studies aiming to disentangle the molecular ramifications of these processes are progressing, we still know little about the genetic basis of evolutionary transitions in reproductive systems. Here we conduct the first comparative analysis of sperm proteomes in Lepidoptera, a group t  ...[more]

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