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Enhanced validation of antibodies for research applications

ABSTRACT: There is a need for standardized validation methods for antibody specificity and selectivity. Recently, five alternative validation “pillars" were proposed to explore the specificity of research antibodies using methods with no need for prior knowledge about the protein target. Here, we show that these principles can be used in a streamlined manner for enhanced validation of research antibodies in Western blot applications. More than 6,000 antibodies were validated with at least one of these strategies involving orthogonal methods, genetic knockdown, recombinant expression, independent antibodies and migration capture mass spectrometry analysis. The results show a path forward for efforts to validate antibodies in an application-specific manner suitable for both providers and users.

INSTRUMENT(S): Bruker Daltonics micrOTOF series

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Andreas Hober

LAB HEAD: Mathias Uhlen

PROVIDER: PXD010178 | Pride | 2018-08-21

REPOSITORIES: Pride

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			Action	DRS
	FE_rt4_b_5of15ul_01_RE2_01_2977.d.zip	Other
	FE_rt4_b_5of15ul_02_RE1_01_2976.d.zip	Other
	FE_rt4_b_5of15ul_03_RD12_01_2975.d.zip	Other
	FE_rt4_b_5of15ul_04_RD11_01_2974.d.zip	Other
	FE_rt4_b_5of15ul_05_RD10_01_2973.d.zip	Other

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