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Identification of potential biomarkers in testicular cancer by SILAC and mass spectrometry

ABSTRACT: This study represents a proteomic resource for testis cancer with quantitative protein expression data for 5236 proteins. By quantitative mass-spectrometry-based proteomics, we compared the two Malignant germ cell tumour cell lines, TCam-2 with seminoma-like characteristics, and NTERA-2, an embryonal carcinoma-like cell line.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Hanibal Bohnenberger

LAB HEAD: Thomas Oellerich

PROVIDER: PXD010275 | Pride | 2019-11-12

REPOSITORIES: Pride

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			Action	DRS
	T_Oellerich_200313_120413_L3_1.raw	Raw
	T_Oellerich_200313_120413_L3_10.raw	Raw
	T_Oellerich_200313_120413_L3_11.raw	Raw
	T_Oellerich_200313_120413_L3_12.raw	Raw
	T_Oellerich_200313_120413_L3_13.raw	Raw

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Publications

Proteomic Comparison of Malignant Human Germ Cell Tumor Cell Lines.

Bremmer Felix F Bohnenberger Hanibal H Küffer Stefan S Oellerich Thomas T Serve Hubert H Urlaub Henning H Strauss Arne A Maatoug Yasmine Y Behnes Carl Ludwig CL Oing Christoph C Radzun Heinz Joachim HJ Ströbel Philipp P Balabanov Stefan S Honecker Friedemann F

Disease markers 20190903

Malignant germ cell tumors (GCT) are the most common malignant tumors in young men between 18 and 40 years. The correct identification of histological subtypes, in difficult cases supported by immunohistochemistry, is essential for therapeutic management. Furthermore, biomarkers may help to understand pathophysiological processes in these tumor types. Two GCT cell lines, TCam-2 with seminoma-like characteristics, and NTERA-2, an embryonal carcinoma-like cell line, were compared by a quantitative ...[more]

PMID: 31565104

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Project description:Testicular germ cell tumors (GCTs) are the most common malignant solid neoplasms in men between the age of 15 and 40 with an increasing incidence seen over the last four decades. Histologically, type II GCT are divided into seminomas (SEM) and non-seminomatous GCT (NSGCT) according to the WHO. NSGCT comprise distinct subentities, e.g. embry-onal carcinomas (EC), yolk sac tumors, choriocarcinomas and teratomas (TER). The distinction between SEM and NSGCT is important because of varying treatment approaches, treatment responses and patients’ prognosis. The treatment of GCTs primarily comprises a radical inguinal orchiectomy of the affected testis. Subsequent cisplatin-based combination chemotherapy is required in metastatic GCTs. The introduction of cisplatin-based com-bination chemotherapy has led to cure rates of up to 90% even in metastatic disease stages. Teratomas are of particular interest, as they are uniformly cisplatin-resistant and sur-gery is the only successful therapy. The number of patients with recurring disease that finally fail several lines of platinum-based chemotherapy is about 3-5% of all GCT patients and about 15% of patients with primary metastatic disease, but they have an exceptionally poor prognosis with a life-expectancy of only a few months. In this study, we compared cisplatin-resistant and cisplatin-sensitive cell lineages of pluripotent NTERA-2 and NCCIT as well as the nullipotent 2102EP cells (with NCCIT deriving from a TP53 mutated mixed mediastinal GCT) by high-resolution mass spectrometric analy-sis (MS) combined with stable isotope labelling with amino acids in cell culture (SILAC). The NTERA-2 and NCCIT cell lines represent EC, which can develop in all other types of NSGCT. Both cell lines represent a very well-studied in vitro model for NSGCTs.

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