Dataset Information

Oryza sativa AM fungi interaction

ABSTRACT: We describe a method that detects membrane proteins from cell type-specific plant plasma membrane subdomains. As the biochemical properties of membrane subdomains are often unknown, general membrane enrichment by continuous sucrose density gradient centrifugation was combined with filter-aided sample preparation, strong cation exchange pre-fractionation and sensitive LTQ Orbitrap Velos-based mass-spectrometry proteomics. This workflow identified plasma membrane proteins, including underrepresented proteins, at an unprecedented level of sensitivity and reproducibility

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Oryza Sativa Subsp. Japonica (rice)

TISSUE(S): Plant Cell, Root

SUBMITTER: Marco Chiapello

LAB HEAD: Uta Paszkowski

PROVIDER: PXD010313 | Pride | 2018-09-24

REPOSITORIES: Pride

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Publications

A rice Serine/Threonine receptor-like kinase regulates arbuscular mycorrhizal symbiosis at the peri-arbuscular membrane.

Roth Ronelle R Chiapello Marco M Montero Héctor H Gehrig Peter P Grossmann Jonas J O'Holleran Kevin K Hartken Denise D Walters Fergus F Yang Shu-Yi SY Hillmer Stefan S Schumacher Karin K Bowden Sarah S Craze Melanie M Wallington Emma J EJ Miyao Akio A Sawers Ruairidh R Martinoia Enrico E Paszkowski Uta U

Nature communications 20181108 1

In terrestrial ecosystems most plant species live in mutualistic symbioses with nutrient-delivering arbuscular mycorrhizal (AM) fungi. Establishment of AM symbioses includes transient, intracellular formation of fungal feeding structures, the arbuscules. A plant-derived peri-arbuscular membrane (PAM) surrounds the arbuscules, mediating reciprocal nutrient exchange. Signaling at the PAM must be well coordinated to achieve this dynamic cellular intimacy. Here, we identify the PAM-specific Arbuscul ...[more]

PMID: 30410018

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