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SseF-SscB Interaction - Revealing the mechanisms of membrane protein export by virulence-associated bacterial secretion systems

ABSTRACT: Bacterial type III secretion systems are able to secrete membrane proteins. In this project we could show that cognate T3SS chaperones are able to bind to the transmembrane segments of T3SS substrates to avoid erroneous targeting to the bacterial inner membrane. Here, we studied the Salmonella SPI-2 T3SS secreted protein SseF and the interaction with its chaperone SscB by in vivo photocrosslinking. LC-MS/MS analysis was done to provide additional evidence that SscB is the interaction partner leading to the upshift of the SseF band in an SDS-PAGE analysis.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Salmonella Enterica Subsp. Enterica Serovar Typhimurium

SUBMITTER: Nicolas Nalpas

LAB HEAD: Samuel Wagner

PROVIDER: PXD010470 | Pride | 2018-10-16

REPOSITORIES: Pride

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			Action	DRS
	20160607_CO_0655SaWa_QBIC_R01_Q0655003AJ.raw	Raw
	20160607_CO_0655SaWa_QBIC_R02_Q0655004AR.raw	Raw
	MaxQuant_Output.zip	Other

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