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Plasma Hirief - In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta

ABSTRACT: Novel method for plasma proteomics and proteogenomics

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Maria Pernemalm

LAB HEAD: Janne Lehtiö

PROVIDER: PXD010899 | Pride | 2019-04-10

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	3-10spike-in_peptidelevelFDR_Galaxy27-out__PSM_table.txt	Txt
	3-10spike-in_peptidelevelFDR_Galaxy34-out__peptide_table.txt	Txt
	3-10spike-in_peptidelevelFDR_Galaxy44-out__protein_table_proteincentric.txt	Txt
	Dummy_NonPhos_PPPFDR_Galaxy49-out__PSM_table.txt	Txt
	Dummy_NonPhos_PPPFDR_Galaxy56-out__peptide_table.txt	Txt

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Publications

In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta.

Pernemalm Maria M Sandberg AnnSofi A Zhu Yafeng Y Boekel Jorrit J Tamburro Davide D Schwenk Jochen M JM Björk Albin A Wahren-Herlenius Marie M Åmark Hanna H Östenson Claes-Göran CG Westgren Magnus M Lehtiö Janne J

eLife 20190408

Here, we present a method for in-depth human plasma proteome analysis based on high-resolution isoelectric focusing HiRIEF LC-MS/MS, demonstrating high proteome coverage, reproducibility and the potential for liquid biopsy protein profiling. By integrating genomic sequence information to the MS-based plasma proteome analysis, we enable detection of single amino acid variants and for the first time demonstrate transfer of multiple protein variants between mother and fetus across the placenta. We ...[more]

PMID: 30958262

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