Proteomics

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Proximity labeling facilitates defining neighborhood proteome of photosystem II oxygen evolution complex in a model cyanobacterium

ABSTRACT: APEX2-based proximity labeling coupled with mass spectrometry have great potential for spatiotemporal identification of proteins proximal to a protein complex of interest. Using this approach it is feasible to define the proteome neighborhood of important protein complexes in a popular photosynthetic model cyanobacterium Synechocystis sp. PCC6803 (hereafter named as Synechocystis). To this end, we developed a robust workflow for APEX2-based proximity labeling in Synechocystis, and used the workflow to identify proteins proximal to the photosystem II (PS II) oxygen evolution complex (OEC) through fusion APEX2 with a luminal OEC subunit, PsbO.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Synechocystis Sp. Pcc 6803

TISSUE(S): Cell Culture

SUBMITTER: Chengcheng Huang  

LAB HEAD: Yingchun Wang

PROVIDER: PXD036113 | Pride | 2023-02-17

REPOSITORIES: Pride

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ELITE_XZ_A-PsbO_1_20210226.raw Raw
ELITE_XZ_A-PsbO_2_20210226.raw Raw
ELITE_XZ_A-PsbO_3_20210226.raw Raw
ELITE_XZ_A-PsbO_BP_1_20210226.raw Raw
ELITE_XZ_A-PsbO_BP_2_20210226.raw Raw
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Publications

Proximity Labeling Facilitates Defining the Proteome Neighborhood of Photosystem II Oxygen Evolution Complex in a Model Cyanobacterium.

Xiao Zhen Z   Huang Chengcheng C   Ge Haitao H   Wang Yan Y   Duan Xiaoxiao X   Wang Gaojie G   Zheng Limin L   Dong Jinghui J   Huang Xiahe X   Zhang Yuanya Y   An Hongyu H   Xu Wu W   Wang Yingchun Y  

Molecular & cellular proteomics : MCP 20221108 12

Ascorbate peroxidase (APEX)-based proximity labeling coupled with mass spectrometry has a great potential for spatiotemporal identification of proteins proximal to a protein complex of interest. Using this approach is feasible to define the proteome neighborhood of important protein complexes in a popular photosynthetic model cyanobacterium Synechocystis sp. PCC6803 (hereafter named as Synechocystis). To this end, we developed a robust workflow for APEX2-based proximity labeling in Synechocystis  ...[more]

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