Dataset Information

Naja annulifera Snake: New Insights into the Venom Components and Pathogenesis of Envenomation

ABSTRACT: Naja annulifera is a dangerous snake that belongs to the Elapidae family. It is found in some of the countries in Sub-Saharan Africa and has caused several accidents in humans and dogs. In this study, we characterized some of the biochemical, toxic and immunogenic properties of N. annulifera venom. We showed that the venom is composed of several proteins, some of which display enzymatic activities, such as phospholipase A2, hyaluronidase, metalloproteinases and serine proteinases. The venom promoted disturbances in the human coagulation system and was cytotoxic to human epidermal cells. Using a mouse model, we showed that the venom promotes local reactions that were reduced with anti-inflammatory drugs. The venom caused systemic inflammation, lung hemorrhage and death. Further, the venom stimulated production of high antibody titers when injected into mice and the antiserum produced was able to inhibit venom-induced death. We therefore conclude that N. annulifera venom contains toxins that play a role in the toxic and inflammatory effects that contribute to the clinical manifestations and death. Moreover, the venom is immunogenic, an important aspect for the production an efficient N. annulifera antivenom.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Naja Annulifera

SUBMITTER: Eduardo Kitano

LAB HEAD: Solange M. T. Serrano

PROVIDER: PXD010962 | Pride | 2019-02-25

REPOSITORIES: Pride

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Naja annulifera Snake: New insights into the venom components and pathogenesis of envenomation.

Silva-de-França Felipe F Villas-Boas Isadora Maria IM Serrano Solange Maria de Toledo SMT Cogliati Bruno B Chudzinski Sonia Aparecida de Andrade SAA Lopes Priscila Hess PH Kitano Eduardo Shigueo ES Okamoto Cinthya Kimori CK Tambourgi Denise V DV

PLoS neglected tropical diseases 20190118 1

<h4>Background</h4>Naja annulifera is a medically important venomous snake occurring in some of the countries in Sub-Saharan Africa. Accidental bites result in severe coagulation disturbances, systemic inflammation and heart damage, as reported in dogs, and death, by respiratory arrest, in humans. Despite the medical importance of N. annulifera, little is known about its venom composition and the pathogenesis of envenomation. In this paper, the toxic, inflammatory and immunogenic properties of N ...[more]

PMID: 30657756

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			Action	DRS
	MaxQuant_txt_files.zip	Other
	N_annul_1_r1.raw	Raw
	N_annul_1_r2.raw	Raw
	N_annul_2_r1.raw	Raw
	N_annul_2_r2.raw	Raw