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Venom profiling of the insular species Bothrops alcatraz: characterization of proteome, glycoproteome, and N-terminome using Terminal Amine Isotopic Labeling of Substrates.

ABSTRACT: In this study we applied mass spectrometric approaches to characterize the proteome and glycoproteome of B. alcatraz venom, and to show for the first time the use of the TAILS method to profile a snake venom N-terminome.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Bothrops Alcatraz

SUBMITTER: Debora Andrade Silva

LAB HEAD: Solange Maria de Toledo Serrano

PROVIDER: PXD021561 | Pride | 2021-01-13

REPOSITORIES: Pride

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			Action	DRS
	Glycoproteome_evidence.txt	Txt
	Glycoproteome_peptides.txt	Txt
	Glycoproteome_proteinGroups.txt	Txt
	ListofRawfiles.xlsx	Xlsx
	N_terminome_analysis_proteinGroups.txt	Txt

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Publications

Venom Profiling of the Insular Species Bothrops alcatraz: Characterization of Proteome, Glycoproteome, and N-Terminome Using Terminal Amine Isotopic Labeling of Substrates.

Andrade-Silva Débora D Zelanis André A Travaglia-Cardoso Silvia R SR Nishiyama Milton Y MY Serrano Solange M T SMT

Journal of proteome research 20210106 2

Bothrops alcatraz, a species endemic to Alcatrazes Islands, is regarded as critically endangered due to its small area of occurrence and the declining quality of its habitat. We recently reported the identification of N-glycans attached to toxins of Bothrops species, showing similar compositions in venoms of the B. jararaca complex (B. jararaca, B. insularis, and B. alcatraz). Here, we characterized B. alcatraz venom using electrophoretic, proteom ...[more]

PMID: 33404253

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The distinct N-terminomes of Bothrops jararaca newborn and adult venoms.

Project description:In this study we applied the Terminal Amine Isotopic Labeling of Substrates (TAILS) technology to characterize the natural N-terminome of newborn and adult Bothrops jararaca venoms.

2021-05-15 | PXD022696 | Pride

De Novo Transcriptome Sequencing for Tetramorium bicarinatum: The first comprehensive venom gland transcriptome analysis from an ant species.

Project description:In order to provide a global insight on the transcripts expressed in the venom gland of the Brazilian ant species Tetramorium bicarinatum and to unveil the potential of its products, high-throughput expressed sequence tags were generated using Illumina paired-end sequencing technology. A total of 212,371,758 pairs of quality-filtered, 100-base-pair Illumina reads were obtained. The de novo assemblies yielded 36,042 contigs for which 27,873 have at least one predicted ORF among which 59.77% produce significant hits in the available databases. The investigation of the reads mapping toxin class revealed a high diversification with the major part consistent with the classical hymenopteran venom protein signature represented by venom allergen (33.3%) followed by a diverse toxin-expression profile including several distinct isoforms of phospholipase A1 and A2, venom serine protease, hyaluronidase, protease inhibitor and secapin. Moreover, our results revealed for the first time the presence of toxin-like peptides that have been previously identified from unrelated venomous animals such as waprin-like (snakes) and agatoxins (spiders and conus). 300 ant specimens from the species Tetramorium bicarinatum were dissected in order to extract the RNA from their venom gland, The whole ant body was used as a reference,

2015-01-08 | E-GEOD-53988 | biostudies-arrayexpress

Qualitative and quantitative analysis of Bothrops jararaca venom gland proteome

Project description:While cellular transcripts encode rich information that provide key features to understand the molecular basis of snake venom variation, their presence/ abundance does not necessary imply/correlate in the translation of a functional (protein) product. In this study we carried out an analysis of the venom gland proteome of Bothrops jararaca taking into account two distinct phases of its ontogenetic development (i.e. newborn and adult specimens) and the marked sexual dimorphism recently reported on its venom proteome. Proteomic data analysis showed wider dynamic range for toxins when comparing to non-toxins and a dynamic proteome rearrangement in cellular proteins upon B. jararaca development. Differentially expressed proteins covered a number of biological pathways related to protein synthesis, including proteins related to transcription and translation, which were found to be significantly higher expressed in the newborn venom gland. Our results showed that the variation in the expression levels of cellular proteins gives rise to an even higher variation in the dynamic range of the expressed toxins. Upon ageing, the molecular constraints related to protein synthesis together with ecological traits would likely have an impact on the toxin repertoire, which, in the case of B. jararaca species, would enable the species to deal with different prey types during its lifespan.

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Project description:Naja annulifera is a dangerous snake that belongs to the Elapidae family. It is found in some of the countries in Sub-Saharan Africa and has caused several accidents in humans and dogs. In this study, we characterized some of the biochemical, toxic and immunogenic properties of N. annulifera venom. We showed that the venom is composed of several proteins, some of which display enzymatic activities, such as phospholipase A2, hyaluronidase, metalloproteinases and serine proteinases. The venom promoted disturbances in the human coagulation system and was cytotoxic to human epidermal cells. Using a mouse model, we showed that the venom promotes local reactions that were reduced with anti-inflammatory drugs. The venom caused systemic inflammation, lung hemorrhage and death. Further, the venom stimulated production of high antibody titers when injected into mice and the antiserum produced was able to inhibit venom-induced death. We therefore conclude that N. annulifera venom contains toxins that play a role in the toxic and inflammatory effects that contribute to the clinical manifestations and death. Moreover, the venom is immunogenic, an important aspect for the production an efficient N. annulifera antivenom.

2019-02-25 | PXD010962 | Pride

Biomarker genes highlight intraspecific and interspecific variations in the responses of Pinus taeda L. and Pinus radiata D. Don to Sirex noctilio F. acid gland secretions

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Transcription profiling of human cell line THP-1 cells exposed to Daboiatoxin and cytokines involved in melioidosis caused by B. pseudomalli

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