Dataset Information

MS on EVs from the YSL of 3dpf zebrafish embryos

ABSTRACT: We set up a zebrafish model to study EVs in vivo by light microscopy, using CD63-pHluorin as a marker. To verify if exosomes from zebrafish have a comparable composition as human exosomes, and to validate the use of CD63 as a marker, we first analysed the compositon of EVs isolated from in vitro cultured fibroblasts (AB.9 ATCC), and could detect the presence of conventional exosome markers, including (zebrafish) CD63. In live embryos, we could track a population of exosomes from their source (the yolk syncytial layer (YSL)) to their final destination by live microscopy. To assess the composition of these exosomes, we dissociated YSL:CD63-pHluorin expressing 3dpf embryos and isolated exosomes from the supernatant and enriched for EVs from the YSL. This was compared to a background of total EVs isolated the control fish (non-expressing).

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Danio Rerio (zebrafish) (brachydanio Rerio)

TISSUE(S): Fibroblast

SUBMITTER: Guillaume Arras

LAB HEAD: Damarys Loew

PROVIDER: PXD011258 | Pride | 2019-04-10

REPOSITORIES: Pride

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Publications

Live Tracking of Inter-organ Communication by Endogenous Exosomes In Vivo.

Verweij Frederik J FJ Revenu Celine C Arras Guillaume G Dingli Florent F Loew Damarys D Pegtel D Michiel DM Follain Gautier G Allio Guillaume G Goetz Jacky G JG Zimmermann Pascale P Herbomel Philippe P Del Bene Filippo F Raposo Graça G van Niel Guillaume G

Developmental cell 20190207 4

Extracellular vesicles (EVs) are released by most cell types but providing evidence for their physiological relevance remains challenging due to a lack of appropriate model organisms. Here, we developed an in vivo model to study EV function by expressing CD63-pHluorin in zebrafish embryos. A combination of imaging methods and proteomic analysis allowed us to study biogenesis, composition, transfer, uptake, and fate of individual endogenous EVs. We identified a subpopulation of EVs with exosome f ...[more]

PMID: 30745143

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