Proteomics

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Mapping the proteome of the synaptic cleft through peroxidase-based reporter protein SynCAM1-HRP

ABSTRACT: Synapses are the brain’s functional units connecting neurons into circuits that underlie memory and behavior. These specialized neuronal junctions are heterogenic in function and molecular composition, reflecting diverse health and disease states. Progress over the past years has shown that trans-synaptic adhesion molecules mediate synapse formation, specification, and differentiation during development. Among the prominently expressed synaptic cleft proteins are SynCAMs, a group of immunoglobulin molecules that engage in homo- and heterophilic interactions and that instruct synapse formation and guide synaptic maturation and that are specific for excitatory synapses. The current study describes the use of peroxidase-mediated proximity labeling to map the proteome of excitatory synapses using SynCAM1 as a reporter protein.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Neuron

SUBMITTER: TuKiet Lam  

LAB HEAD: TuKiet Lam

PROVIDER: PXD011312 | Pride | 2018-12-04

REPOSITORIES: Pride

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Publications

Mapping the Proteome of the Synaptic Cleft through Proximity Labeling Reveals New Cleft Proteins.

Cijsouw Tony T   Ramsey Austin M AM   Lam TuKiet T TT   Carbone Beatrice E BE   Blanpied Thomas A TA   Biederer Thomas T  

Proteomes 20181128 4

Synapses are specialized neuronal cell-cell contacts that underlie network communication in the mammalian brain. Across neuronal populations and circuits, a diverse set of synapses is utilized, and they differ in their molecular composition to enable heterogenous connectivity patterns and functions. In addition to pre- and post-synaptic specializations, the synaptic cleft is now understood to be an integral compartment of synapses that contributes to their structural and functional organization.  ...[more]

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