Dataset Information

In vitro and in vivo proteomic comparison of human neural progenitor cell-induced photoreceptor survival

ABSTRACT: Retinal degenerative diseases are some of the leading causes of blindness with few treatments. Various cell-based therapies have aimed to slow the progression of vision loss by preserving light-sensing photoreceptor cells. A subretinal injection of human neural progenitor cells (hNPCs) into the Royal College of Surgeons (RCS) rat model of retinal degeneration has aided in photoreceptor survival, though the mechanisms are mainly unknown. Identifying the retinal proteomic changes that occur following hNPC treatment will lead to better understanding of neuroprotection. To mimic the retinal environment following hNPC injection, a co-culture system of retinas and hNPCs was developed. Less cell death occurred in RCS retinal tissue co-cultured with hNPCs than in retinas cultured alone, suggesting that hNPCs provide retinal protection in vitro. Comparison of ex vivo and in vivo retinas identified NRF2-mediated oxidative response signaling as an hNPC-induced pathway. This is the first study to compare proteomic changes following treatment with hNPCs in both an ex vivo and in vivo environment, further allowing the use of ex vivo modeling for mechanisms of retinal preservation. Elucidation of the protein changes in the retina following hNPC treatment may lead to the discovery of mechanisms of photoreceptor survival and its therapeutic for clinical applications.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Rattus Norvegicus (rat) Homo Sapiens (human)

TISSUE(S): Cell Suspension Culture, Retina, Neuronal Stem Cell

DISEASE(S): Retinal Degeneration

SUBMITTER: Melissa Jones

LAB HEAD: Shaomei Wang

PROVIDER: PXD011491 | Pride | 2018-12-17

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	10_P30_Day7_Media.mzXML	Mzxml
	10_P30_Day7_Media.pep.xml	Pepxml
	11_P30hNPCs_Day3_Media.mzXML	Mzxml
	11_P30hNPCs_Day3_Media.pep.xml	Pepxml
	12_P30hNPCs-_Day7_Media.mzXML	Mzxml

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Publications

In Vitro and In Vivo Proteomic Comparison of Human Neural Progenitor Cell-Induced Photoreceptor Survival.

Jones Melissa K MK Lu Bin B Chen Dawn Zhaohui DZ Spivia Weston R WR Mercado Augustus T AT Ljubimov Alexander V AV Svendsen Clive N CN Van Eyk Jennifer E JE Wang Shaomei S

Proteomics 20190102 3

Retinal degenerative diseases lead to blindness with few treatments. Various cell-based therapies are aimed to slow the progression of vision loss by preserving light-sensing photoreceptor cells. A subretinal injection of human neural progenitor cells (hNPCs) into the Royal College of Surgeons (RCS) rat model of retinal degeneration has aided in photoreceptor survival, though the mechanisms are mainly unknown. Identifying the retinal proteomic changes that occur following hNPC treatment leads to ...[more]

PMID: 30515959

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