Dataset Information

Global redox proteome and phosphoproteome analysis reveals novel mode of Akt regulation

ABSTRACT: A major knowledge gap in cell signalling is defining the crosstalk between multiple types of post-translational modification (PTM). Here, we take a multi-omic approach (redox proteome, phosphoproteome and total proteome) to delineate signalling networks in adipocytes modulated by reactive oxygen species (ROS) and protein phosphorylation. Our integrative analysis of these datasets revealed widespread and complex crosstalk between oxidative stress-induced cysteine oxidation and phosphorylation-based signalling. In particular, we demonstrate dysregulation in the kinase substrate relationships of Akt, mTOR and AMPK. Furthermore, we identify that Cys60 and Cys77 in the pleckstrin homology (PH) domain of Akt are required for its recruitment to the plasma membrane, its subsequent activation, and its regulation by redox. These multi-omics datasets provide insight into how redox signalling driven by oxidative stress interacts with protein phosphorylation and should serve as a useful resource for dissecting oxidative stress-induced PTMs and understanding their contribution to a variety of diseases.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Adipocyte Cell Line, Preadipocyte

DISEASE(S): Type 2 Diabetes Mellitus

SUBMITTER: Zhiduan Su

LAB HEAD: David E. James

PROVIDER: PXD011525 | Pride | 2019-12-10

REPOSITORIES: Pride

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			Action	DRS
	20140526_Q1_Tim_Drug1_rep1_1.raw	Raw
	20140526_Q1_Tim_Drug1_rep1_10.raw	Raw
	20140526_Q1_Tim_Drug1_rep1_2.raw	Raw
	20140526_Q1_Tim_Drug1_rep1_3.raw	Raw
	20140526_Q1_Tim_Drug1_rep1_4.raw	Raw

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Global redox proteome and phosphoproteome analysis reveals redox switch in Akt.

Su Zhiduan Z Burchfield James G JG Yang Pengyi P Humphrey Sean J SJ Yang Guang G Francis Deanne D Yasmin Sabina S Shin Sung-Young SY Norris Dougall M DM Kearney Alison L AL Astore Miro A MA Scavuzzo Jonathan J Fisher-Wellman Kelsey H KH Wang Qiao-Ping QP Parker Benjamin L BL Neely G Gregory GG Vafaee Fatemeh F Chiu Joyce J Yeo Reichelle R Hogg Philip J PJ Fazakerley Daniel J DJ Nguyen Lan K LK Kuyucak Serdar S James David E DE

Nature communications 20191202 1

Protein oxidation sits at the intersection of multiple signalling pathways, yet the magnitude and extent of crosstalk between oxidation and other post-translational modifications remains unclear. Here, we delineate global changes in adipocyte signalling networks following acute oxidative stress and reveal considerable crosstalk between cysteine oxidation and phosphorylation-based signalling. Oxidation of key regulatory kinases, including Akt, mTOR and AMPK influences the fidelity rather than the ...[more]

PMID: 31792197

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