Proteomics

Dataset Information

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Interference of RNA molecules with protein aggregation in vitro.


ABSTRACT: The analysis of RNA-protein interactomes indicates that the amount of structure in RNA molecules correlates with the capacity of binding to proteins in vitro and in vivo. To validate this observation, we used an in vitro network that mimics the composition of phase-separated RNA granules. The interference of external mRNA molecules on the protein composition of the network was analyzed by quantitative mass spectrometry.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Hela Cell

SUBMITTER: Giulia Calloni  

LAB HEAD: R. Martin Vabulas

PROVIDER: PXD011751 | Pride | 2019-09-13

REPOSITORIES: Pride

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Publications


The combination of high-throughput sequencing and in vivo crosslinking approaches leads to the progressive uncovering of the complex interdependence between cellular transcriptome and proteome. Yet, the molecular determinants governing interactions in protein-RNA networks are not well understood. Here we investigated the relationship between the structure of an RNA and its ability to interact with proteins. Analysing in silico, in vitro and in vivo experiments, we find that the amount of double-  ...[more]

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