Dataset Information

Free mRNA interactome upon heat shock in Hela cells

ABSTRACT: The polypeptide-coding mRNAs are coated by specialized proteins at any stage of their lifecycle. One of few circumstances when free mRNA appears in the cytosol is the disassembly of polysomes during stress-induced shutdown of protein synthesis. Using quantitative mass spectrometry, we identified free RNA interactors in the heat-shocked mammalian cells and reconstituted the protein-RNA association in vitro. RNA-associated proteins displayed higher disorder and larger size, which supports the role of multivalent interactions during the initial phase of the RNA granule formation. Structural features of the free RNA interactors defined them as a subset of RNA-binding proteins. Our results reveal how free RNA can participate in reorganization of cellular functions during proteostasis stress.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Hela Cell

SUBMITTER: Giulia Calloni

LAB HEAD: R. Martin Vabulas

PROVIDER: PXD012945 | Pride | 2019-06-28

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	160512_gc_M3-143_Hsp70-mRNA_PD_control_11A.raw	Raw
	160512_gc_M3-143_Hsp70-mRNA_PD_control_11B.raw	Raw
	160512_gc_M3-143_Hsp70-mRNA_PD_control_11C.raw	Raw
	160512_gc_M3-143_Hsp70-mRNA_PD_control_3A.raw	Raw
	160512_gc_M3-143_Hsp70-mRNA_PD_control_3B.raw	Raw

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Publications

Assembly of Proteins by Free RNA during the Early Phase of Proteostasis Stress.

Alriquet Marion M Martínez-Limón Adrían A Hanspach Gerd G Hengesbach Martin M Tartaglia Gian G GG Calloni Giulia G Vabulas R Martin RM

Journal of proteome research 20190618 7

At any stage of their lifecycle, mRNAs are coated by specialized proteins. One of few circumstances when free mRNA appears in the cytosol is the disassembly of polysomes during the stress-induced shutdown of protein synthesis. Using quantitative mass spectrometry, we sought to identify the free RNA-interacting cellular machinery in heat-shocked mammalian cells. Free RNA-associated proteins displayed higher disorder and larger size, which supports the role of multivalent interactions during the i ...[more]

PMID: 31244213

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