Dataset Information

Polypeptide targeting to distinct areas of the human ER

ABSTRACT: There is at least one additional targeting pathway for proteins to the human endoplasmic reticulum, which involves a cytosolic as well as an ER-membrane resident receptor, belonging to the family of PEX proteins. In contrast to the precursor protein targeting pathways, the additional pathway targets proteins to distinct ER sites and does not target proteins to the Sec61 complex. To characterize substrate proteins of this pathway, we combined siRNA-mediated PEX3-depletion in HeLa cells with label-free quantitative proteomics and and differential protein abundance analysis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Richard Zimmermann

LAB HEAD: Prof. Dr. Bianca Schrul & Prof. Dr. Richard Zimmermann (lab heads)

PROVIDER: PXD012005 | Pride | 2021-12-20

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20180110_coreQEP1_NaNa_LC12-16_SA_Orbi2695Sample01.raw	Raw
	20180110_coreQEP1_NaNa_LC12-16_SA_Orbi2695Sample02.raw	Raw
	20180110_coreQEP1_NaNa_LC12-16_SA_Orbi2695Sample03.raw	Raw
	20180110_coreQEP1_NaNa_LC12-16_SA_Orbi2695Sample04.raw	Raw
	20180110_coreQEP1_NaNa_LC12-16_SA_Orbi2695Sample05.raw	Raw

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Publications

Quantitative Proteomics and Differential Protein Abundance Analysis after the Depletion of PEX3 from Human Cells Identifies Additional Aspects of Protein Targeting to the ER.

Zimmermann Richard R Lang Sven S Lerner Monika M Förster Friedrich F Nguyen Duy D Helms Volkhard V Schrul Bianca B

International journal of molecular sciences 20211201 23

Protein import into the endoplasmic reticulum (ER) is the first step in the biogenesis of around 10,000 different soluble and membrane proteins in humans. It involves the co- or post-translational targeting of precursor polypeptides to the ER, and their subsequent membrane insertion or translocation. So far, three pathways for the ER targeting of precursor polypeptides and four pathways for the ER targeting of mRNAs have been described. Typically, these pathways deliver their substrates to the S ...[more]

PMID: 34884833

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