Dataset Information

Ventriculomics analyses of autopsy AD tissue

ABSTRACT: Very little studies have explored the role played by the specialized choroid plexus epithelial cells and the ependymal cells that line the ventricles. In normal and pathological (AD) ageing, reports of alterations in the blood brain CSF barrier (BCSFB) integrity and membrane transporters, accompanied by deficiencies in CSF production and an enlarged ventricular volume have been documented. The molecular sequelae of events driving these age-related pathological changes remains elusive. Given the pivotal role of the ventricular system in normal brain physiology, in this study we proposed to explore their contributing role towards AD pathogenesis. To address this, we used our state-of-the-art proteomic platform, a liquid chromatography/mass spectrometry (LC-MS) approach coupled with Tandem Mass Tag labeling technology to conduct a detailed characterization and assessment of molecular changes in protein expression levels from isolated tissue from the walls of the ventricles in autopsy AD cases, and two groups of age-matched control cases (non-agenarian’s with no AD pathology, and those with no clinical AD diagnosis but significant amyloid pathology and Braak staging).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain

DISEASE(S): Alzheimer's Disease

SUBMITTER: Joseph Ojo

LAB HEAD: Joseph Ojo

PROVIDER: PXD012059 | Pride | 2021-09-08

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	180111-01Ojo_PoolA_Cyt.raw	Raw
	180111-01Ojo_PoolA_Mem.raw	Raw
	180111-01Ojo_PoolB_Cyt.raw	Raw
	180111-01Ojo_PoolB_Mem.raw	Raw
	180111-01Ojo_PoolC_.msf	Msf

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Publications

Molecular abnormalities in autopsied brain tissue from the inferior horn of the lateral ventricles of nonagenarians and Alzheimer disease patients.

Pearson Andrew A Ajoy Rosa R Crynen Gogce G Reed Jon M JM Algamal Moustafa M Mullan Michael M Purohit Dushyant D Crawford Fiona F Ojo Joseph O JO

BMC neurology 20200827 1

<h4>Background</h4>The ventricular system plays a vital role in blood-cerebrospinal fluid (CSF) exchange and interstitial fluid-CSF drainage pathways. CSF is formed in the specialized secretory tissue called the choroid plexus, which consists of epithelial cells, fenestrated capillaries and the highly vascularized stroma. Very little is currently known about the role played by the ventricles and the choroid plexus tissue in aging and Alzheimer's disease (AD).<h4>Methods</h4>In this study, we use ...[more]

PMID: 32854643

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