Dataset Information

Deuterium-free, Three-plexed Peptide Di-Ethylation for Highly Accurate Quantitative Proteomics

ABSTRACT: The deuterium, frequently used stable isotope in isotopic labeling for quantitative proteomics, could deteriorate the accuracy and precision of proteome quantification owing to the retention time shift of deuterated peptides from the hydrogenated counterpart. Herein, we introduce a novel three-plexed peptide ‘di-ethylation’ using only 13C-isotopologues of acetaldehyde and demonstrate that the accuracy and precision of our method in proteome quantification are significantly superior to the conventional deuterium-based di-methylation labeling in both a single shot and multidimensional LC-MS/MS analysis of HeLa proteome. Furthermore, in time-resolved profiling of Xenopus laevis early embryogenesis, our 3-plexed di-ethylation outperformed isobaric labeling approaches in terms of quantification accuracy or number of protein identification, generating >2 times more differentially expressed proteins. Our cost-effective and highly accurate 3-plexed di-ethylation method could contribute to various types of quantitative proteomics applications, in which three of multiplexity would be sufficient.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

DISEASE(S): Cervix Carcinoma

SUBMITTER: Jae Hun Jung

LAB HEAD: Jaehun Jung

PROVIDER: PXD012117 | Pride | 2019-01-17

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	DE_1_1_1_NCP1C2_J100_0519_01.raw	Raw
	DE_1_1_1_NCP1C2_J100_0519_02.raw	Raw
	DE_1_1_1_NCP1C2_J100_0519_03_RE.raw	Raw
	DE_1_1_1_PRISM_F1_01_NAQ448C1_J100_0618.raw	Raw
	DE_1_1_1_PRISM_F1_02_NAQ448C1_J100_0618.raw	Raw

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Publications

Deuterium-Free, Three-Plexed Peptide Diethylation for Highly Accurate Quantitative Proteomics.

Jung Jaehun J Jeong Kyowon K Choi Yeon Y Kim Sun Ah SA Kim Hyunjoon H Lee Joon Won JW Kim V Narry VN Kim Kwang Pyo KP Kim Jong-Seo JS

Journal of proteome research 20190129 3

The deuterium, a frequently used stable isotope in isotopic labeling for quantitative proteomics, could deteriorate the accuracy and precision of proteome quantification owing to the retention time shift of deuterated peptides from the hydrogenated counterpart. We introduce a novel three-plexed peptide "diethylation" using only <sup>13</sup>C isotopologues of acetaldehyde and demonstrate that the accuracy and precision of our method in proteome quantification are significantly superior to the co ...[more]

PMID: 30638020

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