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Tbeta4 identification CaCo2 cells

ABSTRACT: The paper describes new experiments about thymosin beta 4 nuclear translocation using different in vitro cellular stress in CaCo2 cell line adding new details regarding the role of this peptide during adaptive and defensive response. To this purpose, CaCo2 cells were submitted to different stress treatments, including serum starvation, DMSO and Butyrate treatment. The expression of Tβ4 was studied in culture cells before and after stress treatment in the cytosolic and nuclear fractions, with the use of different apporaches: immunohistochemistry with anti-Tβ4 antibody; mass-spectrometry characterization and quantification, and mRNA analysis.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Colonic Epithelium, Cell Culture

DISEASE(S): Colon Cancer

SUBMITTER: Tiziana Cabras

LAB HEAD: Tiziana Cabras

PROVIDER: PXD012161 | Pride | 2020-06-29

REPOSITORIES: Pride

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			Action	DRS
	Annotated_spectra_MultiConsensus_Tbeta4_CaCo2.zip	Other
	Peak_list_MultiConsensus_Tbeta4_CaCo2.mgf	Mgf
	Search_engine_MultiConsensus.prot_Tbeta4_CaCo2.prot.xml	Xml
	Tbeta4_CaCo2.RAW	Raw
	Tbeta4_Caco2_b.RAW	Raw

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