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Salivary proteomics of sP and sNP rats

ABSTRACT: Sardinian alcohol-preferring (sP) and Sardinina alcohol-non preferring (sNP) rats have been selectively bred for opposite alcohol preference and consumption. The project proposed to identify salivary markers distinguishing the two rat lines possibly correlated to alcohol preference, by a proteomic approach.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Saliva

DISEASE(S): Disease Free

SUBMITTER: Tiziana Cabras

LAB HEAD: Tiziana Cabras

PROVIDER: PXD006997 | Pride | 2017-11-03

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	ANNOTATED-SPECTRA_Bottom-up_proteome_discoverer.7z	Other
	ANNOTATED_SPECTRA_Top-down_proteome_discoverer.7z	Other
	GRP-B_EndoproteinaseV8_peptides_bottom-up_manual.xlsx	Xlsx
	GRP-B_Trypsin_peptides_bottom-up_manual.xlsx	Xlsx
	GRP-B_bottom-up_GluC_Fr_02_220915.raw	Raw

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Publications

Marked Differences in the Submandibular Salivary Proteome between Sardinian Alcohol-Preferring and Sardinian Alcohol-Non Preferring Rats Revealed by an Integrated Top-Down-Bottom-Up Proteomic Platform.

Cabras Tiziana T D'Alessandro Alfredo A Serrao Simone S Isola Raffaella R Iavarone Federica F Vincenzoni Federica F Colombo Giancarlo G Ekström Jörgen J Messana Irene I Castagnola Massimo M

Journal of proteome research 20171116 1

Sardinian alcohol-preferring (sP) and Sardinian alcohol-non preferring (sNP) rats have been selectively bred for opposite alcohol preference and consumption. Aiming to verify possible differences at the proteomics level between sP and sNP rats, we investigated the salivary proteome by a a liquid chromatography-mass spectrometry top-down-bottom-up integrated approach. For this purpose, submandibular saliva was collected from alcohol-naive sP and sNP rats under isoprenaline stimulation. A total of ...[more]

PMID: 29083190

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