Dataset Information

Secretome analysis of Cx43 expressing cells

ABSTRACT: Extracellular matrix remodeling, degradation and glioma cell motility are critical aspects of glioblastoma multiforme (GBM). Despite being a rich source of potential biomarkers and targets for therapeutic advance, the dynamic changes within the extracellular environment that are specific to GBM cell motility have yet to be fully resolved. The gap junction protein connexin43 (Cx43) increases glioma migration and invasion in a variety of in vitro and in vivo models. In this study, the conditioned media of Cx43-expressing C6 rat glioma cells was found to increase the motility of the parental line. Demonstrating the selective engagement of ECM-remodelling pathways, secretome analysis revealed the near-binary expression of osteopontin and matrix metalloproteinase-3 (MMP3).

INSTRUMENT(S): LTQ Orbitrap, 6530B Q-TOF LC/MS

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain

DISEASE(S): Brain Cancer

SUBMITTER: Vincent Chen

LAB HEAD: Vincent Chen

PROVIDER: PXD012175 | Pride | 2019-04-17

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	CHE1908A1MA2-SpectraFile.RAW	Raw
	CHE1908A1MA2-SpectraFile.mgf	Mgf
	CHE1908A1MA2-SpectraFile.mzXML	Mzxml
	CHE1908A2MA2-SpectraFile.RAW	Raw
	CHE1908A2MA2-SpectraFile.mgf	Mgf

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Publications

Cx43-Associated Secretome and Interactome Reveal Synergistic Mechanisms for Glioma Migration and MMP3 Activation.

Aftab Qurratulain Q Mesnil Marc M Ojefua Emmanuel E Poole Alisha A Noordenbos Jenna J Strale Pierre-Olivier PO Sitko Chris C Le Caitlin C Stoynov Nikolay N Foster Leonard J LJ Sin Wun-Chey WC Naus Christian C CC Chen Vincent C VC

Frontiers in neuroscience 20190319

Extracellular matrix (ECM) remodeling, degradation and glioma cell motility are critical aspects of glioblastoma multiforme (GBM). Despite being a rich source of potential biomarkers and targets for therapeutic advance, the dynamic changes occurring within the extracellular environment that are specific to GBM motility have yet to be fully resolved. The gap junction protein connexin43 (Cx43) increases glioma migration and invasion in a variety of <i>in vitro</i> and <i>in vivo</i> models. In thi ...[more]

PMID: 30941001

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