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Effects of CerS4 deletion in murine epidermis on proteome and lipidome

ABSTRACT: Epidermal deletion of the lipid modifying enzyme CerS4 causes epidermal barrier impairments associated with human skin diseases. Proteomes of Wt newborn and P47 mice were compared in order to identify molecular differences between barrier formation and maintenance. Proteomes of epidermal splits from Ctr and CerS4epi-/- at different ages (P21, P33, P47) were compared, to identify the effect of CerS4 on barrier maintenance.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Skin

SUBMITTER: Frederik Tellkamp

LAB HEAD: Carien M. Niessen

PROVIDER: PXD012220 | Pride | 2020-02-27

REPOSITORIES: Pride

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			Action	DRS
	20170206_Q2_JK_ColID_308_ProjID_400_Niessen_Sample_01.raw	Raw
	20170206_Q2_JK_ColID_308_ProjID_400_Niessen_Sample_02.raw	Raw
	20170206_Q2_JK_ColID_308_ProjID_400_Niessen_Sample_03.raw	Raw
	20170309_Q2_UC_ColID_307_ProjID_459_Niessen_Sample_1.raw	Raw
	20170309_Q2_UC_ColID_307_ProjID_459_Niessen_Sample_2.raw	Raw

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Publications

Murine Epidermal Ceramide Synthase 4 Is a Key Regulator of Skin Barrier Homeostasis.

Peters Franziska F Tellkamp Frederik F Brodesser Susanne S Wachsmuth Emmi E Tosetti Bettina B Karow Ulrike U Bloch Wilhelm W Utermöhlen Olaf O Krönke Martin M Niessen Carien M CM

The Journal of investigative dermatology 20200222 10

Epidermal barrier dysfunction is associated with a wide range of highly prevalent inflammatory skin diseases. However, the molecular processes that drive epidermal barrier maintenance are still largely unknown. Here, using quantitative proteomics, lipidomics, and mouse genetics, we characterize epidermal barrier maintenance versus a newly established barrier and functionally identify differential ceramide synthase 4 protein expression as one key difference. We show that epidermal loss of ceramid ...[more]

PMID: 32092351

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