Proteomics

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LC-MS/MS analysis of immunoprecipitation samples of Tetrahymena Mediator complex associated proteins


ABSTRACT: In the germline nucleus of Tetrahymena and other ciliates there occurs a peculiar burst of transcriptional activity during sexual reproduction. A major part of the genome is transcribed into noncoding RNA (ncRNA). This ncRNA serves to spot transposable elements (TEs) and other unwanted DNA sequences and marks them for elimination from the soma of the progeny. ncRNA transcription is remarkable for its extent, its occurrence in a nucleus that bears heterochromatic marks and is incapable of transcribing mRNAs and for its concomitance with meiotic DNA recombination. Here we report two novel factors (Emit1 and Emit2) that promote the micronuclear localization of the transcription Mediator complex to allow this unconventional transcriptional program to occur. Another factor (Rib1) may enhance the transcription activity to TE-rich pericentromeric and telomeric regions. The purpose of the LC-MS/MS analysis of Emit1, Emit2 and Rib1 immunoprecipitation samples is to identify their potential interacting partner proteins.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Tetrahymena Thermophila Sb210

SUBMITTER: Miao Tian  

LAB HEAD: Josef Loidl

PROVIDER: PXD012372 | Pride | 2019-07-11

REPOSITORIES: Pride

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Publications

Non-coding RNA Transcription in Tetrahymena Meiotic Nuclei Requires Dedicated Mediator Complex-Associated Proteins.

Tian Miao M   Mochizuki Kazufumi K   Loidl Josef J  

Current biology : CB 20190704 14


To preserve genome integrity, eukaryotic cells use small RNA-directed mechanisms to repress transposable elements (TEs). Paradoxically, in order to silence TEs, precursors of the small RNAs must be transcribed from TEs. However, it is still poorly understood how these precursors are transcribed from TEs under silenced conditions. In the otherwise transcriptionally silent germline micronucleus (MIC) of Tetrahymena, a burst of non-coding RNA (ncRNA) transcription occurs during meiosis. The transcr  ...[more]

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