Dataset Information

LC-MS/MS analysis of immunoprecipitation samples of Tetrahymena meiotic proteins Melg1, Melg2, Melg3, and Tass1

ABSTRACT: In most eukaryotes, the meiotic chromosomal bouquet (comprising clustered chromosome ends) provides an ordered chromosome arrangement that facilitates pairing and recombination between homologous chromosomes. In the protist Tetrahymena thermophila, the meiotic prophase nucleus stretches enormously and chromosomes assume a bouquet-like arrangement in which telomeres and centromeres are attached to opposite poles of the nucleus. We have identified and characterized three meiosis-specific genes (MELG1-3) that control nuclear elongation and centromere and telomere clustering. Hence, to find out potential interactions, we did LC-MS/MS analysis for Melg1, Melg2, Melg3, and Tass1 (a partner of Melg3) immunoprecipitation samples.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Tetrahymena Thermophila

SUBMITTER: Miao Tian

LAB HEAD: Josef Loidl

PROVIDER: PXD021813 | Pride | 2020-11-23

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20190930_QExHFX1_RSLC1_Tian_Loidl_MFPL_9290_CA_50p.raw	Raw
	20190930_QExHFX1_RSLC1_Tian_Loidl_MFPL_WT_CA_50p.raw	Raw
	20200311_QExHFX1_RSLC1_Tian_Loidl_MFPL_C1_WT_45p.raw	Raw
	20200313_QExHFX1_RSLC1_Tian_Loidl_MFPL_C1_Melg3_45p.raw	Raw
	20200313_QExHFX1_RSLC1_Tian_Loidl_MFPL_S1_Melg3_30p.raw	Raw

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Publications

Spatial constraints on chromosomes are instrumental to meiotic pairing.

Tian Miao M Agreiter Christiane C Loidl Josef J

Journal of cell science 20201130 22

In most eukaryotes, the meiotic chromosomal bouquet (comprising clustered chromosome ends) provides an ordered chromosome arrangement that facilitates pairing and recombination between homologous chromosomes. In the protist <i>Tetrahymena thermophila</i>, the meiotic prophase nucleus stretches enormously, and chromosomes assume a bouquet-like arrangement in which telomeres and centromeres are attached to opposite poles of the nucleus. We have identified and characterized three meiosis-specific g ...[more]

PMID: 33172984

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