Dataset Information

LC-MS/MS analysis of immunoprecipitation samples of Tetrahymena meiotic proteins Mcmd1 and Pamd1

ABSTRACT: Recombinational repair of meiotic DNA double-strand breaks (DSBs) uses the homologous chromosome as a template, although the sister chromatid offers itself as a spatially more convenient substrate. In many organisms, this choice of the homolog rather than the sister is reinforced by the recombination protein Dmc1. We found that, in Tetrahymena, in the absence of two novel proteins, Mcmd1 and Pamd1, Dmc1 is misregulated. Hence, to find out potential interactions, we did LC-MS/MS analysis for Mcmd1 and Pamd1 immunoprecipitation samples.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Tetrahymena Thermophila

SUBMITTER: Thomas Gossenreiter

LAB HEAD: Josef Loidl

PROVIDER: PXD015986 | Pride | 2019-11-19

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20181204_MQ_txt.zip	Other
	20181204_QExHFX4_RSLC3_Tian_Loidl_MFPL_coom_JL3_50p.raw	Raw
	20181204_QExHFX4_RSLC3_Tian_Loidl_MFPL_coom_JL4_50p.raw	Raw
	20190517_MQ_txt.zip	Other
	20190517_QExHFX3_RSLC2_Tian_Loidl_MFPL_coom_PA_70p.raw	Raw

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Publications

An MCM family protein promotes interhomolog recombination by preventing precocious intersister repair of meiotic DSBs.

Tian Miao M Loidl Josef J

PLoS genetics 20191209 12

Recombinational repair of meiotic DNA double-strand breaks (DSBs) uses the homologous chromosome as a template, although the sister chromatid offers itself as a spatially more convenient substrate. In many organisms, this choice is reinforced by the recombination protein Dmc1. In Tetrahymena, the repair of DSBs, which are formed early in prophase, is postponed to late prophase when homologous chromosomes and sister chromatids become juxtaposed owing to tight parallel packing in the thread-shaped ...[more]

PMID: 31815942

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