Proteomics,Multiomics

Dataset Information

0

In situ imaging of the bacterial flagellar motor reveals stable inner- and outer-membrane associated sub-complexes during motor assembly and disassembly


ABSTRACT: Using electron cryo-tomography (ECT), we show that, in three bacterial species, the flagellar motor disassembly process results in stable outer-membrane-embedded sub-complexes. These sub-complexes consist of the periplasmic embellished P- and L-rings, and bend the membrane inward while it remains apparently sealed. Our experiments show that these sub-complexes are more enriched in the cells at high optical-density (OD 600 ). In order to identify candidates for the protein(s) that may play a role in sealing the outer membrane we performed mass spectrometry experiments on membrane vesicles isolated from Shewanella oneidensis MR-1 cells grown to two different optical densities (OD 600 sample 1 ~ 5, OD 600 sample 2 ~ 0.6). Based on certain criteria, we identified few candidate proteins that are overexpressed in cells grown to high OD 600 and that might be involved in sealing the membrane after flagellar motor disassembly.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Shewanella Oneidensis (strain Mr-1)

SUBMITTER: Michael Sweredoski  

LAB HEAD: Spiros Garbis

PROVIDER: PXD012554 | Pride | 2019-05-21

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Mohammed_14Jan19_HOD.raw Raw
Mohammed_14Jan19_LOD.raw Raw
txt.zip Other
Items per page:
1 - 3 of 3

Similar Datasets

2005-02-28 | GSE1981 | GEO
2017-09-22 | PXD005439 | Pride
| 2618337 | ecrin-mdr-crc
2024-04-09 | PXD051103 | Pride
2010-06-10 | E-GEOD-1981 | biostudies-arrayexpress
2017-08-22 | PXD005597 | Pride
2006-12-04 | E-TABM-99 | biostudies-arrayexpress
2014-10-18 | E-GEOD-55199 | biostudies-arrayexpress
2014-05-01 | E-GEOD-51731 | biostudies-arrayexpress
2010-05-16 | E-GEOD-17144 | biostudies-arrayexpress