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CDC14B interactome - The proteomic approach aimed at the identification of interaction partners for the human dual phosphatase and tumor suppressor CDC14B by Co-inmunoprecipitation (Co-IP) experiments. In this approach, CDC14B interactomes from unsynchronized and G2/M enriched cells were compared semi-quantitatively based on spectral counting. This approach yielded the deubiquitylase USP9X as a G2/M-specific CDC14B interactor which has been confirmed by Western-blot (WB).

ABSTRACT: The proteomic approach aimed at the identification of interaction partners for the human dual phosphatase and tumor suppressor CDC14B by Co-inmunoprecipitation (Co-IP) experiments. In this approach, CDC14B interactomes from unsynchronized and G2/M enriched cells were compared semi-quantitatively based on spectral counting. This approach yielded the deubiquitylase USP9X as a G2/M-specific CDC14B interactor which has been confirmed by Western-blot (WB).

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Johannes Gloeckner

LAB HEAD: Christian Johannes Gloeckner

PROVIDER: PXD012732 | Pride | 2020-03-25

REPOSITORIES: pride

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			Action	DRS
	GLO2216A2MA2-40-29070.RAW	Raw
	GLO2216A3MA2-40-29072.RAW	Raw
	GLO2216A4MA2-40-29074.RAW	Raw
	GLO2216_rev1.sf3	Other

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