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HULC interactome MS analysis

ABSTRACT: we report a high throughput strategy to screen for lncRNA interacting proteins in vivo by combining tobramycin affinity purification and mass spectrometric analysis (TOBAP-MS). This method was applied to establish the interactome network for the lncRNA highly-upregulated in liver cancer (HULC).

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Cell Culture

DISEASE(S): Hepatocellular Carcinoma

SUBMITTER: Hao Wang

LAB HEAD: Ruibing Chen

PROVIDER: PXD012927 | Pride | 2020-07-02

REPOSITORIES: Pride

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			Action	DRS
	RChen_HULC1_1.raw	Raw
	RChen_HULC1_10.raw	Raw
	RChen_HULC1_2_.raw	Raw
	RChen_HULC1_3_.raw	Raw
	RChen_HULC1_4.raw	Raw

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Publications

Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.

Wang Chunqing C Li Yongmei Y Yan Shuai S Wang Hao H Shao Xianfeng X Xiao Mingming M Yang Baicai B Qin Guoxuan G Kong Ruirui R Chen Ruibing R Zhang Ning N

Nature communications 20200622 1

Interacting with proteins is a crucial way for long noncoding RNAs (lncRNAs) to exert their biological responses. Here we report a high throughput strategy to characterize lncRNA interacting proteins in vivo by combining tobramycin affinity purification and mass spectrometric analysis (TOBAP-MS). Using this method, we identify 140 candidate binding proteins for lncRNA highly upregulated in liver cancer (HULC). Intriguingly, HULC directly binds to two glycolytic enzymes, lactate dehydrogenase A ( ...[more]

PMID: 32572027

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