Dataset Information

Secretome of Granulicatella adiacens

ABSTRACT: This project investigated the proteins secreted into extracellular medium by the oral bacterium G. adiacens.

INSTRUMENT(S): LTQ FT

ORGANISM(S): Granulicatella Adiacens Atcc 49175

SUBMITTER: Maribasappa Karched

LAB HEAD: Maribasappa Karched

PROVIDER: PXD013000 | Pride | 2019-04-09

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	16D062_Kuwait_400ng.raw	Raw
	16D062_Kuwait_Bacteria_400ng-120min.pdf	Pdf
	LTQ_16D062_Kuwait_400ng.mgf	Mgf

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Publications

Proteomic Analysis and Virulence Assessment of Granulicatella adiacens Secretome.

Karched Maribasappa M Bhardwaj Radhika G RG Tiss Ali A Asikainen Sirkka S

Frontiers in cellular and infection microbiology 20190424

Despite reports on the occurrence of Granulicatella adiacens in infective endocarditis, few mechanistic studies on its virulence characteristics or pathogenicity are available. Proteins secreted by this species may act as determinants of host-microbe interaction and play a role in virulence. Our aim in this study was to investigate and functionally characterize the secretome of G. adiacens. Proteins in the secretome preparation were digested by trypsin and applied to nanoLC-ESI-MS/ ...[more]

PMID: 31069174

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Project description:Malformations of the cardiovascular system are the most common type of birth defect in humans, affecting predominantly the formation of valves and septa. During heart valve and septa formation, cells from the atrio-ventricular canal (AVC) and outflow tract (OFT) regions of the heart undergo an epithelial-to-mesenchymal transformation (EMT) and invade the underlying extracellular matrix to give rise to endocardial cushions. Subsequent maturation of newly formed mesenchyme cells leads to thin stress-resistant leaflets. TWIST1 is a basic helix-loop-helix transcription factor expressed in newly formed mesenchyme cells of the AVC and OFT that has been shown to play roles in cell survival, cell proliferation and differentiation. However, the role and downstream targets of TWIST1 during heart valve formation remain unclear. To identify genes important for heart valve development downstream of Twist1 we performed global gene expression profiling of AVC, OFT, atria and ventricles of the embryonic day 10.5 mouse heart by tag-sequencing (Tag-seq). Using this resource we identified a novel set of 1246 genes, including 201 regulators of transcription, enriched in the valve forming regions of the heart. We compared these genes to a Tag-seq library from the Twist1 null developing valves revealing significant gene expression changes. These changes were consistent with a role of TWIST1 in controlling differentiation of mesenchymal cells following their transformation from endothelium in the mouse. To study the role of TWIST1 at the DNA level we performed chromatin immunoprecipitation and identified novel direct targets of TWIST1 in the developing heart valves. Our findings are consistent with a role for TWIST1 in the differentiation of AVC mesenchyme post-EMT in the mouse, and suggest that TWIST1 exerts its function by direct DNA binding to activate valve specific gene expression. Profiled the AVC, OFT, atria and ventricles of the embryonic day 10.5 mouse heart by tag-sequencing (Tag-seq) (no replicates). We also produced a Tag-seq library from Twist1 null developing valves to reveal the gene expression changes associated with loss of this gene.

Dataset Information

Secretome of Granulicatella adiacens

Dataset's files

Publications

Proteomic Analysis and Virulence Assessment of <i>Granulicatella adiacens</i> Secretome.

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