Proteomics

Dataset Information

0

Docosahexaenoic acid attenuates metabolic dysfunctions induced by lipopolysaccharide in BV-2 microglial cells

ABSTRACT: Microglial cell activation has been linked to many neurodegenerative diseases. Upon stimulation by lipopolysaccharide (LPS), a number of proteins involved in inflammatory and oxidative pathways are activated. Production of nitric oxide has been regarded as a signature marker of inflammatory responses. Our recent studies demonstrated the effects of docosahexaenoic acid (DHA) to inhibit the LPS-induced inflammatory responses in BV-2 microglial cells. DHA also can upregulate the anti-oxidative pathway involving nuclear factor erythroid 2-Like 2 (Nrf2) and synthesis of heme oxygenase-1 (HO-1), a potent anti-oxidative enzyme. In order to further understand the proteins involved, this study used a label-free quantitative proteomics approach to examine effects of DHA and LPS on proteins and signaling pathways in microglial cells.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Microglial Cell

SUBMITTER: Bo Yang  

LAB HEAD: C. Michael Greenlief

PROVIDER: PXD013086 | Pride | 2020-04-20

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
BV2_800ng_DHALPS_1_raw.d.rar Other
BV2_800ng_DHALPS_2_raw.d.rar Other
BV2_800ng_DHALPS_3_raw.d.rar Other
BV2_800ng_DHA_1_raw.d.rar Other
BV2_800ng_DHA_2_raw.d.rar Other
Items per page:
1 - 5 of 38
altmetric image

Publications

Quantitative Proteomics Reveals Docosahexaenoic Acid-Mediated Neuroprotective Effects in Lipopolysaccharide-Stimulated Microglial Cells.

Yang Bo B   Li Runting R   Liu Pei N PN   Geng Xue X   Mooney Brian P BP   Chen Chen C   Cheng Jianlin J   Fritsche Kevin L KL   Beversdorf David Q DQ   Lee James C JC   Sun Grace Y GY   Greenlief C Michael CM  

Journal of proteome research 20200427 6

The high levels of docosahexaenoic acid (DHA) in cell membranes within the brain have led to a number of studies exploring its function. These studies have shown that DHA can reduce inflammatory responses in microglial cells. However, the method of action is poorly understood. Here, we report the effects of DHA on microglial cells stimulated with lipopolysaccharides (LPSs). Data were acquired using the parallel accumulation serial fragmentation method in a hybrid trapped ion mobility-quadrupole  ...[more]

Similar Datasets

Transcriptomics Transcription profiling of blood cells from hypertriglyceridemic men treated with docosahexaenoic acid shows suppression of LDLR and OLR1 gene expression.
2010-02-21 | E-GEOD-20114 | biostudies-arrayexpress
Transcriptomics Transcriptional pattern of E. coli BW25113 and its ptsA, glpK and dhaKLM isogenic mutants growing on DHA (dihydroxyacetone).
2019-06-03 | E-MTAB-7666 | biostudies-arrayexpress
Proteomics Identification of di-Boron cleavage events at Dha residues on recombinant protein in e.coli lysate
2022-05-19 | PXD030233 | Pride
Transcriptomics Whole blood gene expression from overweight women supplemented with quercetin or placebo
2015-11-02 | E-GEOD-74560 | biostudies-arrayexpress
Transcriptomics A Gpr120 Selective Agonist Improves Insulin Resistance and Chronic Inflammation
2014-06-07 | E-GEOD-58282 | biostudies-arrayexpress
Transcriptomics RNA Sequencing Facilitates Quantitative Analysis of BV-2 Transcriptomes
2016-12-04 | GSE75714 | GEO
Genomics miRNA expression profiles and molecular networks in resting and LPS-activated BV-2 microglia - Effect of cannabinoids
2019-01-10 | GSE123262 | GEO
Proteomics Comprehensive proteomic analysis reveals omega-3 fatty acids to counteract endotoxin-stimulated metabolic dysregulation in porcine enterocytes
2023-12-11 | PXD036456 | Pride
Transcriptomics RNAseq of farmed and wild Atlantic salmon fed vegetable oil or fish oil or phospholipid diet from first feeding
2019-10-30 | E-MTAB-8306 | biostudies-arrayexpress
Transcriptomics Microarray and Pathway Analysis Reveal Distinct Mechanisms Underlying Cannabinoid-Mediated Modulation of LPS-Induced Activation of BV-2 Microglial Cells
2015-07-09 | E-GEOD-70689 | biostudies-arrayexpress