Dataset Information

Identification of di-Boron cleavage events at Dha residues on recombinant protein in e.coli lysate

ABSTRACT: Neurokinin-A (NKA) is only active when its C-terminus is amidated. B2(OH2) is thought to interact with Dha and cleave the peptide, leaving the amide on the C-terminus of the left-hand peptide. PeptideID MS/MS experiment was performed in e.coli lysate with overevpressed recombinant NKA-eH3 to prove diBoron activity in biologically relevant context. Overall analysis of the Dha generation and consumption on e.coli lysate was also performed.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Escherichia Coli

SUBMITTER: Yana Demyanenko

LAB HEAD: Ben Davis

PROVIDER: PXD030233 | Pride | 2022-05-19

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	EXPLO_YD1_YZ1_211110_1_1_2_01.raw	Raw
	EXPLO_YD1_YZ1_211110_1_2_2_04.raw	Raw
	EXPLO_YD1_YZ1_211110_1_4_2_07.raw	Raw
	EXPLO_YZ1_YD1_211124_NKA_c_02.raw	Raw
	EXPLO_YZ1_YD1_211124_NKA_s_03.raw	Raw

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Publications

Reductive site-selective atypical <i>C</i>,<i>Z</i>-type/N2-C2 cleavage allows C-terminal protein amidation.

Mollner Tim A TA Giltrap Andrew M AM Zeng Yibo Y Demyanenko Yana Y Buchanan Charles C Oehlrich Daniel D Baldwin Andrew J AJ Anthony Daniel C DC Mohammed Shabaz S Davis Benjamin G BG

Science advances 20220408 14

Biomolecule environments can enhance chemistries with the potential to mediate and modulate self-modification (e.g., self-cleavage). While these enhanced modes are found in certain biomolecules (e.g., RNA ribozymes), it is more rare in proteins. Targeted proteolytic cleavage is vital to physiology, biotechnology, and even emerging therapy. Yet, purely chemically induced methods for the site-selective cleavage of proteins remain scarce. Here, as a proof of principle, we designed and tested a syst ...[more]

PMID: 35394836

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