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Natural occurrence of the reductive glycine pathway in Desulfovibrio desulfuricans strain G11

ABSTRACT: This study was conducted to probe the natural occurrence of a novel CO2-fixation pathway in Desulfovibrio G11.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Desulfovibrio Sp. G11

SUBMITTER: Sjef Boeren

LAB HEAD: Sjef Boeren

PROVIDER: PXD013114 | Pride | 2020-08-17

REPOSITORIES: Pride

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			Action	DRS
	ISA61_Ac1.raw	Raw
	ISA62_Ac2.raw	Raw
	ISA63_Ac3.raw	Raw
	ISA64_Ac4.raw	Raw
	ISA65_Ac5.raw	Raw

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Publications

The reductive glycine pathway allows autotrophic growth of Desulfovibrio desulfuricans.

Sánchez-Andrea Irene I Guedes Iame Alves IA Hornung Bastian B Boeren Sjef S Lawson Christopher E CE Sousa Diana Z DZ Bar-Even Arren A Claassens Nico J NJ Stams Alfons J M AJM

Nature communications 20201009 1

Six CO2 fixation pathways are known to operate in photoautotrophic and chemoautotrophic microorganisms. Here, we describe chemolithoautotrophic growth of the sulphate-reducing bacterium Desulfovibrio desulfuricans (strain G11) with hydrogen and sulphate as energy substrates. Genomic, transcriptomic, proteomic and metabolomic analyses reveal that D. desulfuricans assimilates CO2 via the reductive glycine pathway, a seventh CO2 fixation pathway. In this pathway, CO ...[more]

PMID: 33037220

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Effect of sulfide on growth physiology and gene expression of Desulfovibrio vulgaris Hildenborough

Project description:The effect of sulfide stress on Desulfovibrio vulgaris Hildenborough (DvH) gene expression was determined by comparing the gene expression profiles of DvH under conditions in which sulfide was allowed to accumulate (high sulfide, average concentration 10 mM) against DvH cells grown under conditions in which sulfide was removed by continuous gassing (low sulfide, average concentration 1 mM). High sulfide significantly decreased the instantaneous growth rate constant and final cell density of the culture indicating a decreased bioenergetic fitness. Changes in gene expression caused by exposure to high sulfide were determined using full-genome DvH microarrays. The transcription of ribosomal protein-encoding genes was decreased, in agreement with the lower growth rate of DvH under high sulfide conditions. Interestingly, expression of the gene for DsrD, located downstream of the genes for dissimilatory sulfite reductase (DsrAB) was also strongly down-regulated. In contrast, the expression of many genes involved in iron accumulation, stress response and proteolysis, and chemotaxis were increased. This indicates that high sulfide represents a significant stress condition, in which the bioavailability of metals like iron may be lowered and in which proteins (e.g. metalloenzymes) may need to be refolded, or proteolytically degraded. Overall this leads to a reduced growth rate and less efficient biomass production with available resources. For each condition 2 unique biological samples were hybridized to 4 arrays that each contained duplicate spots. Genomic DNA was used as universal reference.

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Expression analysis of Desulfovibrio africanus strain Walvis Bay

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The reductive glycine pathway allows autotrophic growth of Desulfovibrio desulfuricans.

Project description:Six CO2 fixation pathways are known to operate in photoautotrophic and chemoautotrophic microorganisms. Here, we describe chemolithoautotrophic growth of the sulphate-reducing bacterium Desulfovibrio desulfuricans (strain G11) with hydrogen and sulphate as energy substrates. Genomic, transcriptomic, proteomic and metabolomic analyses reveal that D. desulfuricans assimilates CO2 via the reductive glycine pathway, a seventh CO2 fixation pathway. In this pathway, CO2 is first reduced to formate, which is reduced and condensed with a second CO2 to generate glycine. Glycine is further reduced in D. desulfuricans by glycine reductase to acetyl-P, and then to acetyl-CoA, which is condensed with another CO2 to form pyruvate. Ammonia is involved in the operation of the pathway, which is reflected in the dependence of the autotrophic growth rate on the ammonia concentration. Our study demonstrates microbial autotrophic growth fully supported by this highly ATP-efficient CO2 fixation pathway.

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Comparative transcriptional analysis of Desulfovibrio G20 grown under syntrophic and respiratory condtions

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2013-05-02 | E-GEOD-46547 | biostudies-arrayexpress

Effect of carbon monoxide and the CO sensor CooA on gene expression in Desulfovibrio vulgaris Hildenborough

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2013-08-03 | E-GEOD-49528 | biostudies-arrayexpress

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