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Expression analysis of Desulfovibrio africanus strain Walvis Bay

ABSTRACT: Investigation of partial genome gene expression level changes in a Desulfovibrio africanus during exponential and stationary phase growth in the presence and absence of 5 ug/L Hg2+ (as HgNO3). Desulfovibrio africanus is a known mercury methylating bacteria A 3 chip study using total RNA recovered from three separate cultures of Desulfovibrio africanus with 5 ug/L Hg during exponential phase growth, three seperate cultures of Desulfovibrio africanus with 5 ug/L Hg during stationary phase growth, three cultures of Desulfovibrio africanus without Hg during exponential phase growth, and Desulfovibrio africanus without Hg during stationary phase growth. Each chip measures the expression level of 4,585 genes and intergenic regions from Desulfovibrio africanus strain Walvis Bay on a custom Nimblegen format with 75-mer probes with tiled in 4-plex format.

ORGANISM(S): Desulfovibrio africanus str. Walvis Bay

SUBMITTER: James Moberly 

PROVIDER: E-GEOD-32580 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Role of morphological growth state and gene expression in Desulfovibrio africanus strain Walvis Bay mercury methylation.

Moberly James G JG   Miller Carrie L CL   Brown Steven D SD   Biswas Abir A   Brandt Craig C CC   Palumbo Anthony V AV   Elias Dwayne A DA  

Environmental science & technology 20120420 9

The biogeochemical transformations of mercury are a complex process, with the production of methylmercury, a potent human neurotoxin, repeatedly demonstrated in sulfate- and Fe(III)-reducing as well as methanogenic bacteria. However, little is known regarding the morphology, genes, or proteins involved in methylmercury generation. Desulfovibrio africanus strain Walvis Bay is a Hg-methylating δ-proteobacterium with a sequenced genome and has unusual pleomorphic forms. In this study, a relationshi  ...[more]

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