Dataset Information

Proteomic response to heat shock in males and females of a Baikal endemic amphipod Eulimnogammarus cyaneus (Dyb. 1874) (Crustacea: Amphipoda)

ABSTRACT: In this project we aimed to establish a proteome-based pipeline for a non-model Baikal endemic amphipod species Eulimnogammarus cyaneus (Dybowsky, 1874). We used next-generation transcriptome sequencing to create a database for protein identification and investigated the proteome of E. cyaneus with LC-MS with particular emphasis on sexual dimorphism and response to acute thermal stress. We were able to characterize ca. 1000 protein groups and found male- and female-specific proteins, as well as proteins specific for thermal stress conditions.

INSTRUMENT(S): LTQ Orbitrap XL ETD

ORGANISM(S): Eulimnogammarus Cyaneus

TISSUE(S): Whole Body

SUBMITTER: Polina Drozdova

LAB HEAD: Stefan Kalkhof

PROVIDER: PXD013237 | Pride | 2020-11-19

REPOSITORIES: Pride

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Publications

Proteomics reveals sex-specific heat shock response of Baikal amphipod Eulimnogammarus cyaneus.

Bedulina Daria D Drozdova Polina P Gurkov Anton A von Bergen Martin M Stadler Peter F PF Luckenbach Till T Timofeyev Maxim M Kalkhof Stefan S

The Science of the total environment 20201019

The ancient Lake Baikal is the largest source of liquid freshwater on Earth and home to a unique fauna. Several hundred mostly cold-adapted endemic amphipod species inhabit Baikal, an ecosystem that is already being influenced by global change. In this study, we characterized the core proteome and heat stress-induced changes in a temperature-tolerant endemic amphipod, Eulimnogammarus cyaneus, using a proteogenomic approach (PRIDE dataset PXD013237) to unravel the molecular mechanisms of the obse ...[more]

PMID: 33187699

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