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Role of Has1 RNA helicase in ribosome biogenesis in yeast S. cerevisiae

ABSTRACT: The RNA helicase Has1 is involved in the biogenesis of both small and large ribosomal subunit. How it performs these separate roles is not fully understood. We analysed a detailed composition of RNA-protein complexes purified via Has1.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Martin Kos

LAB HEAD: Martin Koš

PROVIDER: PXD013263 | Pride | 2019-09-09

REPOSITORIES: Pride

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At least two molecules of the RNA helicase Has1 are simultaneously present in pre-ribosomes during ribosome biogenesis.

Gnanasundram Sivakumar Vadivel SV Kos-Braun Isabelle C IC Koš Martin M

Nucleic acids research 20191101 20

The RNA helicase Has1 is involved in the biogenesis of both small and large ribosomal subunits. How it performs these separate roles is not fully understood. Here we provide evidence that at least two molecules of Has1 are temporarily present at the same time in 90S pre-ribosomes. We identified multiple Has1 binding sites in the 18S, 5.8S and 25S rRNAs. We show that while the Has1 catalytic activity is not required for binding to 5.8S/25S region in pre-rRNA, it is essential for binding to 18S si ...[more]

PMID: 31511893

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