Proteomics

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Lysine acetylation of microbiome

ABSTRACT: This project study and comprehensively characterize the lysine acetyltion in the human gut microbiome using antibody-based enrichment strategry and Orbitrap mass spectrometer. The technique has also been applied to study the microbiome in pediatric Crohn's disease and control subjects in order to understand the functional alterations of microbiome in IBD.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Gut Metagenome

TISSUE(S): Gastrointestinal System Mucosa

DISEASE(S): Inflammatory Bowel Disease 1

SUBMITTER: Xu Zhang  

LAB HEAD: Daniel Figeys

PROVIDER: PXD013427 | Pride | 2020-07-08

REPOSITORIES: Pride

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Dataset's files

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Action DRS
IBDmicrobiome_acK_reducedDB_plusHuman.fasta Fasta
Xu_20180317_IBDmicrobiome_acK_HM626DCN_unenrich.raw Raw
Xu_20180317_IBDmicrobiome_acK_HM645DCA_unenrich.raw Raw
Xu_20180317_IBDmicrobiome_acK_HM647DCN_unenrich.raw Raw
Xu_20180317_IBDmicrobiome_acK_HM658DCN_unenrich.raw Raw
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Publications

Widespread protein lysine acetylation in gut microbiome and its alterations in patients with Crohn's disease.

Zhang Xu X   Ning Zhibin Z   Mayne Janice J   Yang Yidai Y   Deeke Shelley A SA   Walker Krystal K   Farnsworth Charles L CL   Stokes Matthew P MP   Couture Jean-François JF   Mack David D   Stintzi Alain A   Figeys Daniel D  

Nature communications 20200817 1

Lysine acetylation (Kac), an abundant post-translational modification (PTM) in prokaryotes, regulates various microbial metabolic pathways. However, no studies have examined protein Kac at the microbiome level, and it remains unknown whether Kac level is altered in patient microbiomes. Herein, we use a peptide immuno-affinity enrichment strategy coupled with mass spectrometry to characterize protein Kac in the microbiome, which successfully identifies 35,200 Kac peptides from microbial or human  ...[more]

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