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Characterisation of a novel glycosylated glutathione transferase of Onchocerca ochengi, closest relative of the human river blindness parasite

ABSTRACT: Filarial nematodes possess glutathione transferases (GSTs), ubiquitous enzymes with potential to detoxify xenobiotic and endogenous substrates, and modulate the host immune system, which may aid worm infection establishment, maintenance and survival in the host. Here we have identified and characterised a sigma class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, O. volvulus, (OvGST1b). O. ochengi native GSTs were purified using a two-step affinity chromatography approach, resolved by 2D and 1D SDS-PAGE and subjected to enzymic deglycosylation revealing the existence of at least four glycoforms. A combination of lectin-blotting and mass spectrometry (MS) analyses of the released N-glycans indicated that OoGST1 contained mainly oligomannose Man5GlcNAc2 structure, but also hybrid- and larger oligommanose-type glycans in a lower proportion. Furthermore, purified OoGST1 showed prostaglandin synthase activity as confirmed by Liquid Chromatography (LC)/MS following a coupled-enzyme assay. This is only the second reported and characterised glycosylated GST and our study highlights its potential role in host-parasite interactions and use in the study of human onchocerciasis.

INSTRUMENT(S): Q-Tof micro

ORGANISM(S): Onchocerca Ochengi (filarial Nematode Worm)

TISSUE(S): Whole Body

SUBMITTER: Stuart Armstrong

LAB HEAD: James LaCourse

PROVIDER: PXD013440 | Pride | 2020-05-26

REPOSITORIES: Pride

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Characterization of a novel glycosylated glutathione transferase of <i>Onchocerca ochengi</i>, closest relative of the human river blindness parasite.

Rose Clair C Praulins Giorgio G Armstrong Stuart D SD Casas-Sanchez Aitor A Davis Jem J Molyneux Gemma G Yunta Cristina C Stead Zenaida Z Prescott Mark M Perally Samirah S Rutter Anne A Makepeace Benjamin L BL La Course E James EJ Acosta-Serrano Alvaro A

Parasitology 20190703 14

Filarial nematodes possess glutathione transferases (GSTs), ubiquitous enzymes with the potential to detoxify xenobiotic and endogenous substrates, and modulate the host immune system, which may aid worm infection establishment, maintenance and survival in the host. Here we have identified and characterized a σ class glycosylated GST (OoGST1), from the cattle-infective filarial nematode Onchocerca ochengi, which is homologous (99% amino acid identity) with an immunodominant GST and potential vac ...[more]

PMID: 31190665

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