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Identification of potential interacting proteins for Drosophila IRP1A, IRP1B, AGBE

ABSTRACT: In an effort to understand the relationship between iron homeostasis and glycogen synthesis in Drosophila, we performed mass spectrometry to identify any candidate interact with IRP1A or IRP1B, the Drosophila orthologs of human IRP1/Aco1 as well as AGBE, the Drosophila ortholog of human GBE1.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Ring Gland, Polytene Cell, Whole Body, Diploid Cell

DISEASE(S): Porphyria,Glycogen Storage Disease Iv

SUBMITTER: Nhan Huynh

LAB HEAD: Nhan Huynh

PROVIDER: PXD013499 | Pride | 2019-10-10

REPOSITORIES: Pride

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Publications

Glycogen branching enzyme controls cellular iron homeostasis via Iron Regulatory Protein 1 and mitoNEET.

Huynh Nhan N Ou Qiuxiang Q Cox Pendleton P Lill Roland R King-Jones Kirst K

Nature communications 20191129 1

Iron Regulatory Protein 1 (IRP1) is a bifunctional cytosolic iron sensor. When iron levels are normal, IRP1 harbours an iron-sulphur cluster (holo-IRP1), an enzyme with aconitase activity. When iron levels fall, IRP1 loses the cluster (apo-IRP1) and binds to iron-responsive elements (IREs) in messenger RNAs (mRNAs) encoding proteins involved in cellular iron uptake, distribution, and storage. Here we show that mutations in the Drosophila 1,4-Alpha-Glucan Branching Enzyme (AGBE) gene cause porphy ...[more]

PMID: 31784520

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