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Identification of histone adducts with glycidamide

ABSTRACT: Identification of protein covalent modifications (adducts) is a challenging task mainly due to the lack of data processing approaches for Adductomics studies. We report a novel strategy to identify covalently modified peptides that consists on LC-MS data preprocessing followed by statistical analysis. This strategy was compared with the standard methodology of adductomics, which relies on producing comprehensive MS/MS data by data dependent acquisition and analysis with proteomics data search engines (Mascot, Global Proteome Machine interface (GPM Fury) that uses X!Tandem, MaxQuant and MSFragger).

INSTRUMENT(S): impact II

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Liver

SUBMITTER: Judit Morello

LAB HEAD: Alexandra MM Antunes

PROVIDER: PXD013683 | Pride | 2019-07-25

REPOSITORIES: Pride

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			Action	DRS
	AdductsMZmine.xlsx	Xlsx
	HepG2_1.pep.xml	Pepxml
	HepG2_12.pep.xml	Pepxml
	HepG2_12_GPM.xml	Xml
	HepG2_12_RD1_01_928.mzXML	Mzxml

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Publications

A Metabolomics-Inspired Strategy for the Identification of Protein Covalent Modifications.

Nunes João J Charneira Catarina C Nunes Carolina C Gouveia-Fernandes Sofia S Serpa Jacinta J Morello Judit J Antunes Alexandra M M AMM

Frontiers in chemistry 20190731

Identification of protein covalent modifications (adducts) is a challenging task mainly due to the lack of data processing approaches for adductomics studies. Despite the huge technological advances in mass spectrometry (MS) instrumentation and bioinformatics tools for proteomics studies, these methodologies have very limited success on the identification of low abundant protein adducts. Herein we report a novel strategy inspired on the metabolomics workflows for the identification of covalently ...[more]

PMID: 31417895

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