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Light-induced orthogonal fragmentation of crosslinked peptides

ABSTRACT: Crosslinking mass spectrometry provides pivotal information on the structure and interaction of proteins. MS-cleavable crosslinkers are regarded as a cornerstone for the analysis of complex mixtures. Yet they fragment under similar conditions as peptides, leading to mixed fragmentation spectra of the crosslinker and peptide. This hampers selecting individual peptides for their independent identification. Here we introduce orthogonal cleavage, using ultraviolet photodissociation (UVPD) to increase crosslinker over peptide fragmentation. We designed and synthesized a crosslinker that can be cleaved at 213 nm in a commercial mass spectrometer configuration. In an analysis of crosslinked E. coli lysate, the crosslinker-to-peptide fragment intensity ratio increases from nearly 1 for a conventionally cleavable crosslinker to 5 for the UVPD cleavable crosslinker. This largely increased the sensitivity of selecting the individual peptides for MS3, even more so with an improved doublet detection algorithm.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human) Escherichia Coli

TISSUE(S): Cell Culture, Blood Serum

SUBMITTER: Lars Kolbowski

LAB HEAD: Juri Rappsilber

PROVIDER: PXD040267 | Pride | 2023-08-28

REPOSITORIES: Pride

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			Action	DRS
	B180615_01_Lumos_AB_DE_160_B1_13_HSA_1.raw	Raw
	B180615_02_Lumos_AB_DE_160_B1_13_HSA_2.raw	Raw
	B180615_03_Lumos_AB_DE_160_B1_13_HSA_3.raw	Raw
	B200421_02_Lumos_AB_DE_165_HSA_UCCL_S1_dualHCD_UVPD_1ms_1.raw	Raw
	B200421_03_Lumos_AB_DE_165_HSA_UCCL_S1_dualHCD_UVPD_5ms_1.raw	Raw

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