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Transcriptomic and phosphoproteomic profiling and metabolite analyses reveal the mechanism of NaHCO3-induced organic acid secretion in grapevine roots

ABSTRACT: Organic acid secretion is a widespread physiological response of plants to alkalinity. However, the features of alkali-induced secretion of organic acid and the underlying mechanism are poorly understood. Herein, it was indicated that oxalate was the main organic acid synthesized and secreted in vine roots, and acetate synthesis and malate secretion were also promoted under NaHCO3 stress. NaHCO3 stress enhanced H+ efflux rate of vine roots, which was related to plasma membrane H+-ATPase activity. Transcriptomic profiling revealed that carbohydrate metabolism was the most significantly altered biological process under NaHCO3 stress; a total of seven genes related to organic acid metabolism were significantly altered, including two PEPCs and PEPCKs. Additionally, the expression levels of five ABC transporters, particularly ABCB19, as well as two malate transporter ALMT2s, were largely upregulated by NaHCO3 stress. Phosphoproteomic profiling demonstrated that the altered phosphoproteins were primarily related to binding, catalytic activity and transporter activity in light of their molecular functions. The phosphorylation levels of PEPC3, two plasma membrane H+-ATPases 4 and ABC transporters ABCB19 and PDR12 were significantly increased. Additionally, the inhibition of ethylene synthesis and perception completely blocked NaHCO3-induced organic acid secretion, while the inhibition of IAA synthesis reduced NaHCO3-induced organic acid secretion. Collectively, our results demonstrated oxalate as the main organic acid under alkali stress and the necessity of ethylene in mediating organic acid secretion, as well as further identified several candidate genes and phosphoproteins responsible for organic acid metabolism and secretion.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Vitis Vinifera (grape)

TISSUE(S): Root

SUBMITTER: lihuan Qin

LAB HEAD: Yuxin Yao

PROVIDER: PXD013746 | Pride | 2019-11-11

REPOSITORIES: Pride

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Transcriptomic and phosphoproteomic profiling and metabolite analyses reveal the mechanism of NaHCO<sub>3</sub>-induced organic acid secretion in grapevine roots.

Xiang Guangqing G Ma Wanyun W Gao Shiwei S Jin Zhongxin Z Yue Qianyu Q Yao Yuxin Y

BMC plant biology 20190903 1

<h4>Background</h4>Organic acid secretion is a widespread physiological response of plants to alkalinity. However, the characteristics and underlying mechanism of the alkali-induced secretion of organic acids are poorly understood.<h4>Results</h4>Oxalate was the main organic acid synthesized and secreted in grapevine (a hybrid of Vitis amurensis, V. berlandieri and V. riparia) roots, while acetate synthesis and malate secretion were also promoted under NaHCO<sub>3</sub> stress. NaHCO<sub>3</sub> ...[more]

PMID: 31481025

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Transcriptomic and phosphoproteomic profiling and metabolite analyses reveal the mechanism of NaHCO3-induced organic acid secretion in grapevine roots

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Transcriptomic and phosphoproteomic profiling and metabolite analyses reveal the mechanism of NaHCO<sub>3</sub>-induced organic acid secretion in grapevine roots.

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