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Proteomics of Bordetella pertussis whole-cell and acellular vaccines

ABSTRACT: Bordetella pertussis is the etiological agent of whooping cough, a bacterial infection of especially children, which may be fatal without treatment. In frame of studies to investigate putative effects of vaccination on host-pathogen interaction and clonal distribution of strains, in addition to Corynebacterium diphtheriae and Clostridium tetani toxoid vaccines, also whole-cell and acellular pertussis vaccines were analyzed by mass spectrometry.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Clostridium Tetani E88 Bordetella Pertussis (strain Tohama I / Atcc Baa-589 / Nctc 13251) Corynebacterium Diphtheriae Nctc 13129

TISSUE(S): Bacterial Protein

DISEASE(S): Bordetella Parapertussis Whooping Cough,Tetanus,Diphtheria

SUBMITTER: Jens Möller

LAB HEAD: Andreas Burkovski

PROVIDER: PXD013804 | Pride | 2019-05-14

REPOSITORIES: Pride

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			Action	DRS
	aPv.msf	Msf
	aPv.pep.xml	Pepxml
	aPv.raw	Raw
	aPv.xlsx	Xlsx
	wPv_run1.msf	Msf

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Project description:Diphtheria toxoid vaccines are among the oldest and safest vaccines known. The basic principle of production is the inactivation of purified diphtheria toxin by formaldehyde cross-linking, which converts the potentially fatal toxin in a completely harmless protein aggregate, which is still immunogenic. Since in addition to diphtheria toxin also other proteins might be secreted by Corynebacterium diphtheriae, we assumed that diphtheria toxoid might not be the only component present in the vaccine. Therefore, we established a protocol to reverse formaldehyde cross-linking and carried out mass spectrometric analyses. Different secreted, membrane-associated and cytoplasmic proteins of C. diphtheriae were detected in several vaccine preparations from across the world. Western blot analyses indicated that these are immunogenic and may therefore support protection against C. diphtheriae. Furthermore, we could show that the vaccines also induce antibodies directed against diphtheria toxin secreted by Corynebacterium ulcerans, an emerging pathogen evoking diphtheria-like illness and skin infections.

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