Whole cellular and secreted proteins of C. diphtheriae ISS3319 grown in rich medium
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion
ORGANISM(S): Corynebacterium Diphtheriae Bv. Mitis Str. Iss 3319
DISEASE(S): Diphtheria
SUBMITTER: 
Jens Möller
LAB HEAD: Andreas Burkovski
PROVIDER: PXD036352 | Pride | 2023-04-13
REPOSITORIES: Pride
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| BHI_R1.msf | Msf | |||
| BHI_R1.raw | Raw | |||
| BHI_R2.msf | Msf | |||
| BHI_R2.raw | Raw | |||
| BHI_R3.msf | Msf |
Items per page: 5 1 - 5 of 13 |
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Project description:Introduced for mass immunization in the 1920s, vaccines against diphtheria are among the oldest and safest vaccines known. The basic principle of their production is the inactivation of purified diphtheria toxin by formaldehyde cross-linking, which converts the potentially fatal toxin in a completely harmless protein aggregate, which is still immunogenic. Since in addition to diphtheria toxin also other proteins may be secreted by Corynebacterium diphtheriae during cultivation, we assumed that diphtheria toxoid might not be the only component present in the vaccine. In this study, we established a protocol to reverse formaldehyde cross-linking and carried out mass spectrometric analyses. Different secreted, membrane-associated and cytoplasmic proteins of C. diphtheriae were detected in several vaccine preparations from across the world. Western blot analyses indicated that at least some of these are immunogenic and may therefore support protection against C. diphtheriae. In frame of this study, we could show that the C. diphtheriae toxoid vaccines also induce antibodies directed against diphtheria toxin secreted by Corynebacterium ulcerans, an emerging pathogen which is outnumbering C. diphtheriae as cause of diphtheria-like illness in Western Europe, but do not induce immune reaction against other C. ulcerans proteins.
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