Proteomics

Dataset Information

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Role of the DapA protein in iron homeostasis and the metabolism of cyanide in jewelery wastewaters


ABSTRACT: Quantitative free labeling proteomics to elucidate the role of the DapA protein in iron metabolism and the assimilation of cyanide in the bacterium Pseudomonas pseudoalcaligenes. Integration, validation and discussion of results using other non-proteomic techniques such as qRT-PCR and determination of key metals.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Bacteria Pseudomonas Pseudoalcaligenes Cect 5344

SUBMITTER: Alfonso Olaya Abril  

LAB HEAD: María Dolores Roldán Ruiz

PROVIDER: PXD014027 | Pride | 2020-02-18

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
DapA-NH4-1.raw Raw
DapA-NH4-2.raw Raw
DapA-NH4-3.raw Raw
DapA-R-1.raw Raw
DapA-R-2.raw Raw
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Publications

Role of the Dihydrodipicolinate Synthase DapA1 on Iron Homeostasis During Cyanide Assimilation by the Alkaliphilic Bacterium <i>Pseudomonas pseudoalcaligenes</i> CECT5344.

Olaya-Abril Alfonso A   Pérez María Dolores MD   Cabello Purificación P   Martignetti Diego D   Sáez Lara Paloma LP   Luque-Almagro Víctor Manuel VM   Moreno-Vivián Conrado C   Roldán María Dolores MD  

Frontiers in microbiology 20200123


Cyanide is a toxic compound widely used in mining and jewelry industries, as well as in the synthesis of many different chemicals. Cyanide toxicity derives from its high affinity for metals, which causes inhibition of relevant metalloenzymes. However, some cyanide-degrading microorganisms like the alkaliphilic bacterium <i>Pseudomonas pseudoalcaligenes</i> CECT5344 may detoxify hazardous industrial wastewaters that contain elevated cyanide and metal concentrations. Considering that iron availabi  ...[more]

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