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Clavibacter nebraskensis secretome analysis

ABSTRACT: The project aimed to study the secretome profile of the Goss's bacterial wilt and blight disease of corn caused by the phytobacterial pathogen Clavibacter nebraskensis (Cn). The experiment was carried out in Vitro using two Cn isolates (A highly aggressive isolate Cmn14-5-1 and a weakly aggressive DOAB232) from Manitoba, Canada grown in two different xylem sap media (Host xylem sap from corn (CXS), and non-host xylem sap from tomato (TXS), besides M9 medium.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Clavibacter Nebraskensis Ncppb 2581

SUBMITTER: Atta Soliman

LAB HEAD: Fouad Daayf

PROVIDER: PXD014510 | Pride | 2021-09-08

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	CXS-Cmn14-5-1R1.dat	Other
	CXS-Cmn14-5-1R1.mgf	Mgf
	CXS-Cmn14-5-1R2.dat	Other
	CXS-Cmn14-5-1R2.mgf	Mgf
	CXS-Cmn14-5-1R3.dat	Other

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Publications

Secretome Analysis of Clavibacter nebraskensis Strains Treated with Natural Xylem Sap In Vitro Predicts Involvement of Glycosyl Hydrolases and Proteases in Bacterial Aggressiveness.

Soliman Atta A Rampitsch Christof C Tambong James T JT Daayf Fouad F

Proteomes 20210109 1

The Gram-positive bacterium Clavibacter nebraskensis (Cn) causes Goss's wilt and leaf blight on corn in the North American Central Plains with yield losses as high as 30%. Cn strains vary in aggressiveness on corn, with highly aggressive strains causing much more serious symptoms and damage to crops. Since Cn inhabits the host xylem, we investigated differences in the secreted proteomes of Cn strains to determine whether these could account for phenotypic differences in aggressiveness. Hi ...[more]

PMID: 33435483

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Changes in the Proteome of Xylem Sap in Brassica oleracea in Response to Fusarium oxysporum Stress

Project description:Fusarium oxysporum f. sp. conlutinans (Foc) is a serious root-invading and xylem-colonizing fungus that causes yellowing in Brassica oleracea. To comprehensively understand the interaction between F. oxysporum and B. oleracea, composition of the xylem sap proteome of the non-infected and Foc-infected plants was investigated in both resistant and susceptible cultivars using liquid chromatography-tandem mass spectrometry (LC-MS/MS) after in-solution digestion of xylem sap proteins. Whole genome sequencing of Foc was carried out and generated a predicted Foc protein database. The predicted Foc protein database was then combined with the public B. oleracea and B. rapa protein databases downloaded from Uniprot and used for protein identification. About 200 plant proteins were identified in the xylem sap of susceptible and resistant plants. Comparison between the non-infected and Foc-infected samples revealed that Foc infection causes changes to the protein composition in B. oleracea xylem sap where repressed proteins accounted for a greater proportion than those of induced in both the susceptible and resistant reactions. The analysis on the proteins with concentration change >=2 fold indicated a large portion of up- and down-regulated proteins were those acting on carbohydrates. Proteins with leucine-rich repeats and legume lectin domains were mainly induced in both resistant and susceptible system, so was the case of thaumatins. Twenty-five Foc proteins were identified in the infected xylem sap and ten of them were cysteine-containing secreted small proteins that are good candidates for virulence and/or avirulence effectors. The findings of differential response of protein contents in the xylem sap between the non-infected and Foc-infected samples as well as the Foc candidate effectors secreted in xylem provide valuable insights into B. oleracea-Foc interactions.

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Multi‐omics analysis of xylem sap uncovers dynamic modulation of poplar defenses by ammonium and nitrate

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