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Clavibacter nebraskensis secretome analysis

ABSTRACT: The project aimed to study the secretome profile of the Goss's bacterial wilt and blight disease of corn caused by the phytobacterial pathogen Clavibacter nebraskensis (Cn). The experiment was carried out in Vitro using two Cn isolates (A highly aggressive isolate Cmn14-5-1 and a weakly aggressive DOAB232) from Manitoba, Canada grown in two different xylem sap media (Host xylem sap from corn (CXS), and non-host xylem sap from tomato (TXS), besides M9 medium.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Clavibacter Nebraskensis Ncppb 2581

SUBMITTER: Atta Soliman

LAB HEAD: Fouad Daayf

PROVIDER: PXD014510 | Pride | 2021-09-08

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	CXS-Cmn14-5-1R1.dat	Other
	CXS-Cmn14-5-1R1.mgf	Mgf
	CXS-Cmn14-5-1R2.dat	Other
	CXS-Cmn14-5-1R2.mgf	Mgf
	CXS-Cmn14-5-1R3.dat	Other

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Publications

Secretome Analysis of Clavibacter nebraskensis Strains Treated with Natural Xylem Sap In Vitro Predicts Involvement of Glycosyl Hydrolases and Proteases in Bacterial Aggressiveness.

Soliman Atta A Rampitsch Christof C Tambong James T JT Daayf Fouad F

Proteomes 20210109 1

The Gram-positive bacterium Clavibacter nebraskensis (Cn) causes Goss's wilt and leaf blight on corn in the North American Central Plains with yield losses as high as 30%. Cn strains vary in aggressiveness on corn, with highly aggressive strains causing much more serious symptoms and damage to crops. Since Cn inhabits the host xylem, we investigated differences in the secreted proteomes of Cn strains to determine whether these could account for phenotypic differences in aggressiveness. Hi ...[more]

PMID: 33435483

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Changes in the Proteome of Xylem Sap in Brassica oleracea in Response to Fusarium oxysporum Stress

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Multi‐omics analysis of xylem sap uncovers dynamic modulation of poplar defenses by ammonium and nitrate

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