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Xylem sap shotgun proteomics from Mn-excess tomato plants

ABSTRACT: The aim of this work was to study the effects of Mn excess on the protein profile of the xylem sap of tomato (Solanum lycopersicum), with the aim of elucidating plant response mechanisms to these nutritional stresses. Tomato was chosen as a model plant because the tomato genome has been published and this plant species has adequate root pressure and turgid stems that permit xylem sap sampling in sufficient amounts by de-topping. The high- throughput shotgun analysis has permitted to identify and quantitate a large number of low abundance proteins in the tomato xylem sap.

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Solanum Lycopersicum

TISSUE(S): Xylem

SUBMITTER: Laura Ceballos-Laita

LAB HEAD: Javier Abadia

PROVIDER: PXD021973 | Pride | 2020-12-10

REPOSITORIES: Pride

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			Action	DRS
	PEAK.mgf	Mgf
	PeptidesMASCOT.xlsx	Xlsx
	QUANT.xlsx	Xlsx
	SEARCH.xml	Xml
	Sample01.RAW	Raw

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Publications

Effects of Excess Manganese on the Xylem Sap Protein Profile of Tomato (Solanum lycopersicum) as Revealed by Shotgun Proteomic Analysis.

Ceballos-Laita Laura L Gutierrez-Carbonell Elain E Takahashi Daisuke D Lonsdale Andrew A Abadía Anunciación A Doblin Monika S MS Bacic Antony A Uemura Matsuo M Abadía Javier J López-Millán Ana Flor AF

International journal of molecular sciences 20201123 22

Metal toxicity is a common problem in crop species worldwide. Some metals are naturally toxic, whereas others such as manganese (Mn) are essential micro-nutrients for plant growth but can become toxic when in excess. Changes in the composition of the xylem sap, which is the main pathway for ion transport within the plant, is therefore vital to understanding the plant's response(s) to metal toxicity. In this study we have assessed the effects of exposure of tomato roots to excess Mn on the protei ...[more]

PMID: 33238539

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Xylem sap shotgun proteomics from Fe- and Mn-deficient and Mn-toxic tomato plants

Project description:The aim of this work was to study the effects of Fe and Mn deficiencies and Mn toxicity on the protein profile of the xylem sap of tomato (Solanum lycopersicum), with the aim of elucidating plant response mechanisms to these nutritional stresses. Tomato was chosen as a model plant because the tomato genome has been published and this plant species has adequate root pressure and turgid stems that permit xylem sap sampling in sufficient amounts by de-topping. The high-throughput shotgun analysis has permitted to identify and quantitate a large number of low abundance proteins in the tomato xylem sap.

2017-11-30 | PXD007517 | Pride

Root shotgun proteomics from Fe- and Mn-deficient and Mn-toxic tomato plants

Project description:The aim of this work was to study the effects of Fe and Mn deficiencies and Mn toxicity on the protein profile of the root of tomato (Solanum lycopersicum), with the aim of elucidating plant response mechanisms to these nutritional stresses. Tomato was chosen as a model plant because the tomato genome has been published. The high-throughput shotgun analysis has permitted to identify and quantitate a large number of low abundance proteins in the tomato root. Protein identification was carried out using the Mascot search engine and the non-redundant databases NCBInr and ITAG v2.3.

2018-08-06 | PXD008326 | Pride

Clavibacter nebraskensis secretome analysis

Project description:The project aimed to study the secretome profile of the Goss's bacterial wilt and blight disease of corn caused by the phytobacterial pathogen Clavibacter nebraskensis (Cn). The experiment was carried out in Vitro using two Cn isolates (A highly aggressive isolate Cmn14-5-1 and a weakly aggressive DOAB232) from Manitoba, Canada grown in two different xylem sap media (Host xylem sap from corn (CXS), and non-host xylem sap from tomato (TXS), besides M9 medium.

2021-09-08 | PXD014510 | Pride

Tomato xylem proteome composition following infection by Fusarium oxysporum

Project description:The influence of during colonization by Fusarium oxysporum f. sp. Lycopersici secreted effector proteins on the proteome of the xylem sap of tomato plants was investigated using a label-free quantitative proteomics approach. A comparison was made between plants inoculated with either a mock control, a non-effector knockout control, Fusarium oxysporum Fol007 wildtype and four Fol007 single effector protein knockout strains. Specific effects on the relative abundance of certain proteins of the xylem sap occurred for the different knockout strains next to a core set of 24 differentially accumulated proteins which may provide insights into the mechanisms of promoting infection for each of the tested effector proteins.

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Changes in the Proteome of Xylem Sap in Brassica oleracea in Response to Fusarium oxysporum Stress

Project description:Fusarium oxysporum f. sp. conlutinans (Foc) is a serious root-invading and xylem-colonizing fungus that causes yellowing in Brassica oleracea. To comprehensively understand the interaction between F. oxysporum and B. oleracea, composition of the xylem sap proteome of the non-infected and Foc-infected plants was investigated in both resistant and susceptible cultivars using liquid chromatography-tandem mass spectrometry (LC-MS/MS) after in-solution digestion of xylem sap proteins. Whole genome sequencing of Foc was carried out and generated a predicted Foc protein database. The predicted Foc protein database was then combined with the public B. oleracea and B. rapa protein databases downloaded from Uniprot and used for protein identification. About 200 plant proteins were identified in the xylem sap of susceptible and resistant plants. Comparison between the non-infected and Foc-infected samples revealed that Foc infection causes changes to the protein composition in B. oleracea xylem sap where repressed proteins accounted for a greater proportion than those of induced in both the susceptible and resistant reactions. The analysis on the proteins with concentration change >=2 fold indicated a large portion of up- and down-regulated proteins were those acting on carbohydrates. Proteins with leucine-rich repeats and legume lectin domains were mainly induced in both resistant and susceptible system, so was the case of thaumatins. Twenty-five Foc proteins were identified in the infected xylem sap and ten of them were cysteine-containing secreted small proteins that are good candidates for virulence and/or avirulence effectors. The findings of differential response of protein contents in the xylem sap between the non-infected and Foc-infected samples as well as the Foc candidate effectors secreted in xylem provide valuable insights into B. oleracea-Foc interactions.

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Microarray analysis of Lycopersicon esculentum plants subjected to tomato spotted wilt virus inoculation, western flower thrips infestation and a combination of virus and thrips

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Multi‐omics analysis of xylem sap uncovers dynamic modulation of poplar defenses by ammonium and nitrate

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