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Ovarian fluid proteome variation associates with sperm swimming speed in an externally fertilizing fish

ABSTRACT: Ovarian fluid was collected from 6 adult female Chinook salmon during their annual spawning run. Two replicates were analyzed for each ovarian fluid sample fraction using 80 and 100 minute gradients, respectively. Protein abundances were estimated using iTRAQ.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Oncorhynchus Tshawytscha

TISSUE(S): Coelomic Fluid

SUBMITTER: Kirill Borziak

LAB HEAD: Steve Dorus

PROVIDER: PXD014551 | Pride | 2021-04-17

REPOSITORIES: Pride

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			Action	DRS
	13022_IEFa_Fr1.RAW	Raw
	13022_IEFa_Fr1.mgf	Mgf
	13022_IEFa_Fr1.pride.mgf.gz	Mgf
	13022_IEFa_Fr10.RAW	Raw
	13022_IEFa_Fr10.mgf	Mgf

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Ovarian fluid proteome variation associates with sperm swimming speed in an externally fertilizing fish.

Johnson Sheri L SL Borziak Kirill K Kleffmann Torsten T Rosengrave Patrice P Dorus Steve S Gemmell Neil J NJ

Journal of evolutionary biology 20201025 12

Sperm velocity is a key trait that predicts the outcome of sperm competition. By promoting or impeding sperm velocity, females can control fertilization via postcopulatory cryptic female choice. In Chinook salmon, ovarian fluid (OF), which surrounds the ova, mediates sperm velocity according to male and female identity, biasing the outcome of sperm competition towards males with faster sperm. Past investigations have revealed proteome variation in OF, but the specific components of OF that diffe ...[more]

PMID: 33034086

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